biostudies-literatureKim BKHealth Technology R&amp;D GrantHealth Technology R&D GrantNational Research FoundationKorea Research Institute of Bioscience and Biotechnology212https://www.ebi.ac.uk/biostudies/studies/S-EPMC9241269biostudies-literatureUnknown41(1)<h4>Background</h4>Identifying biomarkers related to the diagnosis and treatment of gastric cancer (GC) has not made significant progress due to the heterogeneity of tumors. Genes involved in histological classification and genetic correlation studies are essential to develop an appropriate treatment for GC.<h4>Methods</h4>In vitro and in vivo lentiviral shRNA library screening was performed. The expression of Synaptotagmin (SYT11) in the tumor tissues of patients with GC was confirmed by performing Immunohistochemistry, and the correlation between the expression level and the patient's survival rate was analyzed. Phospho-kinase array was performed to detect Jun N-terminal kinase (JNK) phosphorylation. SYT11, JNK, and MKK7 complex formation was confirmed by western blot and immunoprecipitaJournal of experimental & clinical cancer research : CRSynaptotagmin 11 scaffolds MKK7-JNK signaling process to promote stem-like molecular subtype gastric cancer oncogenesis.PMC9241269KGM5192221NRF-2019R1A2C1087492HI13C2162Park HKim SYJung HLee JKweon DHKim BKKim DMByun JEHwang MAYeom YICheong JHKang MJKang MKim SKIm JYWon MPark KCfalseSynaptotagmin 11 scaffolds MKK7-JNK signaling process to promote stem-like molecular subtype gastric cancer oncogenesis.<h4>Background</h4>Identifying biomarkers related to the diagnosis and treatment of gastric cancer (GC) has not made significant progress due to the heterogeneity of tumors. Genes involved in histological classification and genetic correlation studies are essential to develop an appropriate treatment for GC.<h4>Methods</h4>In vitro and in vivo lentiviral shRNA library screening was performed. The expression of Synaptotagmin (SYT11) in the tumor tissues of patients with GC was confirmed by performing Immunohistochemistry, and the correlation between the expression level and the patient's survival rate was analyzed. Phospho-kinase array was performed to detect Jun N-terminal kinase (JNK) phosphorylation. SYT11, JNK, and MKK7 complex formation was confirmed by western blot and immunoprecipita2022-01-01T00:00:00Z2022 Jun2026-05-27T21:39:11.496Z2022-07-08T11:35:45.229ZS-EPMC92412693576884210.1186/s13046-022-02420-3