biostudies-literatureUnknown13Kim YTComplete genome sequence analysis of <i>Bifidobacterium longum</i> subsp. <i>longum</i> BCBL-583 isolated from a Korean female fecal sample showed no virulence factor or antibiotic resistance gene, suggesting human safety. In addition, this strain has oxygen and heat tolerance genes for food processing, and cholesterol reduction and mucin adhesion-related genes were also found. For <i>in vivo</i> evaluations, a high fat diet (HFD) mouse model was used, showing that BCBL-583 administration to the model (HFD-583) reduced the total cholesterol and LDL-cholesterol in the blood and decreased pro-inflammatory cytokines but increased anti-inflammatory cytokines, substantiating its cholesterol reduction and anti-inflammation activities. Subsequent microbiome analysis of the fecal samples from the Frontiers in microbiology886934https://www.ebi.ac.uk/biostudies/studies/S-EPMC9247516biostudies-literature<i>In vivo</i> Trial of <i>Bifidobacterium longum</i> Revealed the Complex Network Correlations Between Gut Microbiota and Health Promotional Effects.PMC9247516Kwon JGKim CHCho JHShin YSKim YTKim HBLee JHfalse<i>In vivo</i> Trial of <i>Bifidobacterium longum</i> Revealed the Complex Network Correlations Between Gut Microbiota and Health Promotional Effects.Complete genome sequence analysis of <i>Bifidobacterium longum</i> subsp. <i>longum</i> BCBL-583 isolated from a Korean female fecal sample showed no virulence factor or antibiotic resistance gene, suggesting human safety. In addition, this strain has oxygen and heat tolerance genes for food processing, and cholesterol reduction and mucin adhesion-related genes were also found. For <i>in vivo</i> evaluations, a high fat diet (HFD) mouse model was used, showing that BCBL-583 administration to the model (HFD-583) reduced the total cholesterol and LDL-cholesterol in the blood and decreased pro-inflammatory cytokines but increased anti-inflammatory cytokines, substantiating its cholesterol reduction and anti-inflammation activities. Subsequent microbiome analysis of the fecal samples from the 2022-01-01T00:00:00Z20222026-05-10T03:02:20.779Z2022-07-08T00:08:49.215ZS-EPMC92475163578342110.3389/fmicb.2022.886934