biostudies-literatureFu YNational Basic Research Program of ChinaNational Institutes of HealthNIH HHSe13257https://www.ebi.ac.uk/biostudies/studies/S-EPMC9251052biostudies-literatureUnknown55(7)<h4>Objectives</h4>CDC45 is the core component of CMG (CDC45-MCMs-GINS) complex that plays important role in the initial step of DNA replication in eukaryotic cells. The expression level of cdc45 is under the critical control for the accurate cell cycle progression. Loss-of-function of cdc45 has been demonstrated to inhibit cell proliferation and leads to cell death due to the inhibition of DNA replication and G1-phase arrest. An increasing of CDC45 inhibits cell proliferation as well. Nevertheless, a systematic analysis of the effect of high dose of CDC45 on cell physiology and behaviors is unclear. In the present study, we aimed to investigate the effects and mechanisms of high dose of CDC45 on cell behaviors.<h4>Materials and methods</h4>We overexpressed cdc45 in cultured cell lines, CiCell proliferationHigh abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.PMC9251052P40OD0185372018YFD09007052019YFE0190900P40 OD018537Lv ZFan YFu YKong DDong BfalseHigh abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.<h4>Objectives</h4>CDC45 is the core component of CMG (CDC45-MCMs-GINS) complex that plays important role in the initial step of DNA replication in eukaryotic cells. The expression level of cdc45 is under the critical control for the accurate cell cycle progression. Loss-of-function of cdc45 has been demonstrated to inhibit cell proliferation and leads to cell death due to the inhibition of DNA replication and G1-phase arrest. An increasing of CDC45 inhibits cell proliferation as well. Nevertheless, a systematic analysis of the effect of high dose of CDC45 on cell physiology and behaviors is unclear. In the present study, we aimed to investigate the effects and mechanisms of high dose of CDC45 on cell behaviors.<h4>Materials and methods</h4>We overexpressed cdc45 in cultured cell lines, Ci2022-01-01T00:00:00Z2022 Jul2026-06-18T03:54:19.833Z2022-07-20T09:43:41.636ZS-EPMC92510523564273310.1111/cpr.13257