{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nagano M"],"funding":["Exploratory Research for Advanced Technology","NIAID NIH HHS","NINDS NIH HHS","Open Philanthropy Project","HFSP","NCI NIH HHS","National Institutes of Health","Japan Society for the Promotion of Science"],"pagination":["e110600"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9251848"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(13)"],"pubmed_abstract":["Germ cells are unique in engendering totipotency, yet the mechanisms underlying this capacity remain elusive. Here, we perform comprehensive and in-depth nucleome analysis of mouse germ-cell development in vitro, encompassing pluripotent precursors, primordial germ cells (PGCs) before and after epigenetic reprogramming, and spermatogonia/spermatogonial stem cells (SSCs). Although epigenetic reprogramming, including genome-wide DNA de-methylation, creates broadly open chromatin with abundant enhancer-like signatures, the augmented chromatin insulation safeguards transcriptional fidelity. These insulatory constraints are then erased en masse for spermatogonial development. Notably, despite distinguishing epigenetic programming, including global DNA re-methylation, the PGCs-to-spermatogonia/S"],"journal":["The EMBO journal"],"pubmed_title":["Nucleome programming is required for the foundation of totipotency in mammalian germline development."],"pmcid":["PMC9251848"],"funding_grant_id":["RGP0057/2018","R01 NS111997","JP20H05387","P01 CA196539","CA196539","JP18H02613","22H04920","JPMJER1104","2018‐193685","NS111997","17H06098","R01 AI118891"],"pubmed_authors":["Yokobayashi S","Mirny LA","Yabuta Y","Shimizu S","Ishikura Y","Nosaka Y","Garcia BA","Yamamura A","Coradin M","Kawahira N","Murakawa Y","Tachibana K","Hiraoka Y","Peters JM","Okamoto I","Meehan K","Stocsits R","Imoto Y","Yamamoto T","Hu B","Saitou M","Ohta H","Mizuta K","Ikeda H","Wutz G","Kojima Y","Kasahara T","Majewski J","Nagano M","Umemura F"],"additional_accession":[]},"is_claimable":false,"name":"Nucleome programming is required for the foundation of totipotency in mammalian germline development.","description":"Germ cells are unique in engendering totipotency, yet the mechanisms underlying this capacity remain elusive. Here, we perform comprehensive and in-depth nucleome analysis of mouse germ-cell development in vitro, encompassing pluripotent precursors, primordial germ cells (PGCs) before and after epigenetic reprogramming, and spermatogonia/spermatogonial stem cells (SSCs). Although epigenetic reprogramming, including genome-wide DNA de-methylation, creates broadly open chromatin with abundant enhancer-like signatures, the augmented chromatin insulation safeguards transcriptional fidelity. These insulatory constraints are then erased en masse for spermatogonial development. Notably, despite distinguishing epigenetic programming, including global DNA re-methylation, the PGCs-to-spermatogonia/S","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-05-27T22:42:38.595Z","creation":"2024-12-03T19:33:51.579Z"},"accession":"S-EPMC9251848","cross_references":{"pubmed":["35703121"],"doi":["10.15252/embj.2022110600"]}}