{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ju MK"],"funding":["Ministry of Health and Welfare","National Research Foundation of Korea"],"pagination":["e53492"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9253748"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(7)"],"pubmed_abstract":["Genome instability is one of the leading causes of gastric cancers. However, the mutational landscape of driver genes in gastric cancer is poorly understood. Here, we investigate somatic mutations in 25 Korean gastric adenocarcinoma patients using whole-exome sequencing and show that PWWP2B is one of the most frequently mutated genes. PWWP2B mutation correlates with lower cancer patient survival. We find that PWWP2B has a role in DNA double-strand break repair. As a nuclear protein, PWWP2B moves to sites of DNA damage through its interaction with UHRF1. Depletion of PWWP2B enhances cellular sensitivity to ionizing radiation (IR) and impairs IR-induced foci formation of RAD51. PWWP2B interacts with MRE11 and participates in homologous recombination via promoting DNA end-resection. Taken tog"],"journal":["EMBO reports"],"pubmed_title":["PWWP2B promotes DNA end resection and homologous recombination."],"pmcid":["PMC9253748"],"funding_grant_id":["NRF‐2021R1A6A1A03038890","NRF‐2018R1A2B2003129","NRF‐2014M3C9A2064688","HA17C0054","NRF‐2019R1A2C2089746"],"pubmed_authors":["Lee S","Zang DY","Kim B","Kim H","Park SY","Lee JR","Soh JS","Myung K","Choi Y","Kim KC","An S","Chung HJ","Cho JW","Jung E","Seo J","Kim HS","Kang HS","Kim BJ","Lee JY","Lim H","Kim Y","Ju MK","Sul HJ","Choi BY","Kim TM"],"additional_accession":[]},"is_claimable":false,"name":"PWWP2B promotes DNA end resection and homologous recombination.","description":"Genome instability is one of the leading causes of gastric cancers. However, the mutational landscape of driver genes in gastric cancer is poorly understood. Here, we investigate somatic mutations in 25 Korean gastric adenocarcinoma patients using whole-exome sequencing and show that PWWP2B is one of the most frequently mutated genes. PWWP2B mutation correlates with lower cancer patient survival. We find that PWWP2B has a role in DNA double-strand break repair. As a nuclear protein, PWWP2B moves to sites of DNA damage through its interaction with UHRF1. Depletion of PWWP2B enhances cellular sensitivity to ionizing radiation (IR) and impairs IR-induced foci formation of RAD51. PWWP2B interacts with MRE11 and participates in homologous recombination via promoting DNA end-resection. Taken tog","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-19T02:37:09.697Z","creation":"2025-04-07T12:58:06.674Z"},"accession":"S-EPMC9253748","cross_references":{"pubmed":["35582821"],"doi":["10.15252/embr.202153492"]}}