biostudies-literatureUnknown5(2)Palassin P<b>Aim:</b> The transcription factor RIP140 (receptor interacting protein of 140 kDa) is involved in intestinal tumorigenesis. It plays a role in the control of microsatellite instability (MSI), through the regulation of <i>MSH2</i> and <i>MSH6</i> gene expression. The aim of this study was to explore its effect on the expression of <i>POLK</i>, the gene encoding the specialized translesion synthesis (TLS) DNA polymerase κ known to perform accurate DNA synthesis at microsatellites. <b>Methods:</b> Different mouse models and engineered human colorectal cancer (CRC) cell lines were used to analyze by RT-qPCR, while Western blotting and luciferase assays were used to elucidate the role of RIP140 on <i>POLK</i> gene expression. Published DNA microarray datasets were reanalyzed. The <i>in vitroCancer drug resistance (Alhambra, Calif.)401-414https://www.ebi.ac.uk/biostudies/studies/S-EPMC9255241biostudies-literatureRIP140 regulates <i>POLK</i> gene expression and the response to alkylating drugs in colon cancer cells.PMC9255241Castet-Nicolas ACavailles VGyorffy BLapierre MTeyssier CPillaire MJHoffmann JSPalassin PBonnet SJalaguier SfalseRIP140 regulates <i>POLK</i> gene expression and the response to alkylating drugs in colon cancer cells.<b>Aim:</b> The transcription factor RIP140 (receptor interacting protein of 140 kDa) is involved in intestinal tumorigenesis. It plays a role in the control of microsatellite instability (MSI), through the regulation of <i>MSH2</i> and <i>MSH6</i> gene expression. The aim of this study was to explore its effect on the expression of <i>POLK</i>, the gene encoding the specialized translesion synthesis (TLS) DNA polymerase κ known to perform accurate DNA synthesis at microsatellites. <b>Methods:</b> Different mouse models and engineered human colorectal cancer (CRC) cell lines were used to analyze by RT-qPCR, while Western blotting and luciferase assays were used to elucidate the role of RIP140 on <i>POLK</i> gene expression. Published DNA microarray datasets were reanalyzed. The <i>in vitro2022-01-01T00:00:00Z20222025-04-04T10:43:36.948Z2022-07-23T10:14:45.014ZS-EPMC92552413580038010.20517/cdr.2021.133