biostudies-literatureSidiras PFonds Erasme pour la Recherche Médicale’Radio-télévision belge de la Communauté française (RTBF)Fonds de la recherche scientifique-FNRSKing Baudouin Foundation2826-2834https://www.ebi.ac.uk/biostudies/studies/S-EPMC9258537biostudies-literatureUnknown61(7)<h4>Objectives</h4>Anti-carbamylated protein antibodies (anti-CarPAs) are present in RA sera and have been associated with erosive disease. The exact targets of anti-CarPAs in vivo are currently not well known; we used a proteomic approach on serum and SF of RA patients to assess the human carbamylome and to identify carbamylated autoantigens as potential biomarkers in early RA.<h4>Methods</h4>Mass spectrometry was performed on SF and serum from RA patients. Carbamylated proteins present in both sample types were selected as candidate autoantigens for the establishment of ELISAs. A cohort of early RA patients was tested for positivity for specific anti-CarPAs.<h4>Results</h4>Eleven novel carbamylated proteins were identified, and five were selected as potential autoantigens for detection oRheumatology (Oxford, England)Human carbamylome description identifies carbamylated α2-macroglobulin and hemopexin as two novel autoantigens in early rheumatoid arthritis.PMC9258537CAP48Lechanteur JImbault VSidiras PDurez PRasschaert JSokolova TGangji VCommuni DfalseHuman carbamylome description identifies carbamylated α2-macroglobulin and hemopexin as two novel autoantigens in early rheumatoid arthritis.<h4>Objectives</h4>Anti-carbamylated protein antibodies (anti-CarPAs) are present in RA sera and have been associated with erosive disease. The exact targets of anti-CarPAs in vivo are currently not well known; we used a proteomic approach on serum and SF of RA patients to assess the human carbamylome and to identify carbamylated autoantigens as potential biomarkers in early RA.<h4>Methods</h4>Mass spectrometry was performed on SF and serum from RA patients. Carbamylated proteins present in both sample types were selected as candidate autoantigens for the establishment of ELISAs. A cohort of early RA patients was tested for positivity for specific anti-CarPAs.<h4>Results</h4>Eleven novel carbamylated proteins were identified, and five were selected as potential autoantigens for detection o2022-01-01T00:00:00Z2022 Jul2025-04-25T20:59:43.067Z2022-07-23T08:44:53.935ZS-EPMC92585373478840910.1093/rheumatology/keab838