biostudies-literatureAzali MAUniversiti Sains Malaysia98https://www.ebi.ac.uk/biostudies/studies/S-EPMC9259773biostudies-literatureUnknown20(1)<h4>Background</h4>The baculovirus expression vector system has been developed for expressing a wide range of proteins, including enzymes, glycoproteins, recombinant viruses, and vaccines. The availability of the SARS-CoV-2 genome sequence has enabled the synthesis of SARS-CoV2 proteins in a baculovirus-insect cell platform for various applications. The most cloned SARS-CoV-2 protein is the spike protein, which plays a critical role in SARS-CoV-2 infection. It is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD). Non-structural proteins (Nsps), another recombinant SARS-CoV-2 protein generated by the baculovirus expression vector system (BEV), are used in the identification of new medications or the repurposing of existing therapies for the treatment Journal, genetic engineering & biotechnologyApplication of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review.PMC92597731001/PPSP/8011025Hussain FAMohamed SAzali MAShamsuddin SJohan MFHarun AfalseApplication of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review.<h4>Background</h4>The baculovirus expression vector system has been developed for expressing a wide range of proteins, including enzymes, glycoproteins, recombinant viruses, and vaccines. The availability of the SARS-CoV-2 genome sequence has enabled the synthesis of SARS-CoV2 proteins in a baculovirus-insect cell platform for various applications. The most cloned SARS-CoV-2 protein is the spike protein, which plays a critical role in SARS-CoV-2 infection. It is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD). Non-structural proteins (Nsps), another recombinant SARS-CoV-2 protein generated by the baculovirus expression vector system (BEV), are used in the identification of new medications or the repurposing of existing therapies for the treatment 2022-01-01T00:00:00Z2022 Jul2025-04-04T12:40:42.564Z2025-04-04T12:40:42.564ZS-EPMC92597733579296610.1186/s43141-022-00368-7