{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bergman DT"],"funding":["NHGRI NIH HHS","Novo Nordisk Fonden","NIGMS NIH HHS"],"pagination":["176-184"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9262863"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["607(7917)"],"pubmed_abstract":["Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters<sup>1</sup>. A proposed model for this specificity is that promoters have sequence-encoded preferences for certain enhancers, for example, mediated by interacting sets of transcription factors or cofactors<sup>2</sup>. This 'biochemical compatibility' model has been supported by observations at individual human promoters and by genome-wide measurements in Drosophila<sup>3-9</sup>. However, the degree to which human enhancers and promoters are intrinsically compatible has not yet been systematically measured, and how their activities combine to control RNA expression remains unclear. Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active "],"journal":["Nature"],"pubmed_title":["Compatibility rules of human enhancer and promoter sequences."],"pmcid":["PMC9262863"],"funding_grant_id":["R00 HG009917","T32 GM007753","T32 GM008313","R35 HG011324","K99 HG009917","T32 HG000044","NNF21SA0072102"],"pubmed_authors":["Jagoda E","Grossman SR","Jones TR","Ray J","Nguyen TH","Bergman DT","Liu V","Lander ES","Nasser J","Kang HY","Rios A","Siraj L","Fulco CP","Engreitz JM","Kane M"],"additional_accession":[]},"is_claimable":false,"name":"Compatibility rules of human enhancer and promoter sequences.","description":"Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters<sup>1</sup>. A proposed model for this specificity is that promoters have sequence-encoded preferences for certain enhancers, for example, mediated by interacting sets of transcription factors or cofactors<sup>2</sup>. This 'biochemical compatibility' model has been supported by observations at individual human promoters and by genome-wide measurements in Drosophila<sup>3-9</sup>. However, the degree to which human enhancers and promoters are intrinsically compatible has not yet been systematically measured, and how their activities combine to control RNA expression remains unclear. Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-19T13:31:15.259Z","creation":"2025-04-19T13:31:15.259Z"},"accession":"S-EPMC9262863","cross_references":{"pubmed":["35594906"],"doi":["10.1038/s41586-022-04877-w"]}}