biostudies-literatureLu MScience and technology project of Shaanxi Tobacco BureauKey Research and Development Program of Shaanxi ProvinceScience and Technology Program of Shaanxi Academy of SciencesNatural Science Foundation of Gansu ProvinceUniversities Innovation Foundation of Gansu Province4259https://www.ebi.ac.uk/biostudies/studies/S-EPMC9268043biostudies-literatureUnknown27(13)Blue mold caused by Penicillium expansum is one of the most common apple diseases, and it is becoming a serious threat in apple production. The strain <i>Bacillus amyloliquefaciens</i> Ba168 showed high levels of antimicrobial activity in our previous study. To analyze the antimicrobial protein of Ba168, a high-resolution LC-MS/MS proteomic analysis was performed. A total of 1155 proteins were identified from 5233 unique peptides. A total of 16 potential antimicrobial-activity-related proteins were identified; 10 of these proteins have direct antimicrobial effects, while 6 of these proteins are associated with the formation of antimicrobial substances. Then, an antifungal protein of Ba168 was isolated and purified by the sequential chromatography of DEAE Bio-sep FF anion exchange and SephaMolecules (Basel, Switzerland)Analysis and Evaluation of the Flagellin Activity of <i>Bacillus amyloliquefaciens</i> Ba168 Antimicrobial Proteins against <i>Penicillium expansum</i>.PMC92680432022K-02-0521JR7RA8202020NY-0462021B-117SCYC202009Lu MSun PTong ZMa YChen YGuo DLi LAn DfalseAnalysis and Evaluation of the Flagellin Activity of <i>Bacillus amyloliquefaciens</i> Ba168 Antimicrobial Proteins against <i>Penicillium expansum</i>.Blue mold caused by Penicillium expansum is one of the most common apple diseases, and it is becoming a serious threat in apple production. The strain <i>Bacillus amyloliquefaciens</i> Ba168 showed high levels of antimicrobial activity in our previous study. To analyze the antimicrobial protein of Ba168, a high-resolution LC-MS/MS proteomic analysis was performed. A total of 1155 proteins were identified from 5233 unique peptides. A total of 16 potential antimicrobial-activity-related proteins were identified; 10 of these proteins have direct antimicrobial effects, while 6 of these proteins are associated with the formation of antimicrobial substances. Then, an antifungal protein of Ba168 was isolated and purified by the sequential chromatography of DEAE Bio-sep FF anion exchange and Sepha2022-01-01T00:00:00Z2022 Jul2025-04-22T09:47:00.949Z2025-04-05T23:15:24.768ZS-EPMC92680433580750310.3390/molecules27134259