{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang L"],"funding":["National Natural Science Foundation of China","Innovation and Entrepreneurship Talent Plan of Jiangsu Province","Natural Science Foundation of Jiangsu Province"],"pagination":["292"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9284797"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["<h4>Background</h4>Improved understanding of the stemness regulation mechanism in intrahepatic cholangiocarcinoma (ICC) could identify targets and guidance for adjuvant transarterial chemoembolization (TACE).<h4>Methods</h4>TCGA database was excavated to identify the ICC stemness-associated genes. The pro-stemness effect of target genes was further analyzed by sphere formation assay, qRT-PCR, western blot, flow cytometric analysis, IHC, CCK8 assay and metabolomic analysis. Based on multivariate analysis, a nomogram for ICC patients with adjuvant TACE was established and our result was further confirmed by a validation cohort. Finally, the effect of dietary methionine intervention on chemotherapy was estimated by in vivo experiment and clinical data.<h4>Results</h4>In this study, we identif"],"journal":["Stem cell research & therapy"],"pubmed_title":["A gene signature is critical for intrahepatic cholangiocarcinoma stem cell self-renewal and chemotherapeutic response."],"pmcid":["PMC9284797"],"funding_grant_id":["82002533","BK20191062"],"pubmed_authors":["Qian Y","Guo H","Zhang Y","Zhang X","Xu D","Xia Q","Huang L","Sha M","Hu R","Kong X"],"additional_accession":[]},"is_claimable":false,"name":"A gene signature is critical for intrahepatic cholangiocarcinoma stem cell self-renewal and chemotherapeutic response.","description":"<h4>Background</h4>Improved understanding of the stemness regulation mechanism in intrahepatic cholangiocarcinoma (ICC) could identify targets and guidance for adjuvant transarterial chemoembolization (TACE).<h4>Methods</h4>TCGA database was excavated to identify the ICC stemness-associated genes. The pro-stemness effect of target genes was further analyzed by sphere formation assay, qRT-PCR, western blot, flow cytometric analysis, IHC, CCK8 assay and metabolomic analysis. Based on multivariate analysis, a nomogram for ICC patients with adjuvant TACE was established and our result was further confirmed by a validation cohort. Finally, the effect of dietary methionine intervention on chemotherapy was estimated by in vivo experiment and clinical data.<h4>Results</h4>In this study, we identif","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-05T12:40:12.473Z","creation":"2024-12-04T12:09:18.296Z"},"accession":"S-EPMC9284797","cross_references":{"pubmed":["35841118"],"doi":["10.1186/s13287-022-02988-9"]}}