biostudies-literatureHuang LNational Natural Science Foundation of ChinaInnovation and Entrepreneurship Talent Plan of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province292https://www.ebi.ac.uk/biostudies/studies/S-EPMC9284797biostudies-literatureUnknown13(1)<h4>Background</h4>Improved understanding of the stemness regulation mechanism in intrahepatic cholangiocarcinoma (ICC) could identify targets and guidance for adjuvant transarterial chemoembolization (TACE).<h4>Methods</h4>TCGA database was excavated to identify the ICC stemness-associated genes. The pro-stemness effect of target genes was further analyzed by sphere formation assay, qRT-PCR, western blot, flow cytometric analysis, IHC, CCK8 assay and metabolomic analysis. Based on multivariate analysis, a nomogram for ICC patients with adjuvant TACE was established and our result was further confirmed by a validation cohort. Finally, the effect of dietary methionine intervention on chemotherapy was estimated by in vivo experiment and clinical data.<h4>Results</h4>In this study, we identifStem cell research & therapyA gene signature is critical for intrahepatic cholangiocarcinoma stem cell self-renewal and chemotherapeutic response.PMC928479782002533BK20191062Qian YGuo HZhang YZhang XXu DXia QHuang LSha MHu RKong XfalseA gene signature is critical for intrahepatic cholangiocarcinoma stem cell self-renewal and chemotherapeutic response.<h4>Background</h4>Improved understanding of the stemness regulation mechanism in intrahepatic cholangiocarcinoma (ICC) could identify targets and guidance for adjuvant transarterial chemoembolization (TACE).<h4>Methods</h4>TCGA database was excavated to identify the ICC stemness-associated genes. The pro-stemness effect of target genes was further analyzed by sphere formation assay, qRT-PCR, western blot, flow cytometric analysis, IHC, CCK8 assay and metabolomic analysis. Based on multivariate analysis, a nomogram for ICC patients with adjuvant TACE was established and our result was further confirmed by a validation cohort. Finally, the effect of dietary methionine intervention on chemotherapy was estimated by in vivo experiment and clinical data.<h4>Results</h4>In this study, we identif2022-01-01T00:00:00Z2022 Jul2025-04-05T12:40:12.473Z2024-12-04T12:09:18.296ZS-EPMC92847973584111810.1186/s13287-022-02988-9