{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rutigliano HM"],"funding":["NICHD NIH HHS"],"pagination":["e13520"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9285385"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["87(3)"],"pubmed_abstract":["<h4>Problem</h4>A significant rate of spontaneous abortion is observed in cattle pregnancies produced by somatic cell nuclear transfer (SCNT). Major histocompatibility complex class I (MHC-I) proteins are abnormally expressed on the surface of trophoblast cells from SCNT conceptuses.<h4>Method of study</h4>MHC-I homozygous compatible (n = 9), homozygous incompatible (n = 8), and heterozygous incompatible (n = 5) pregnancies were established by SCNT. Eight control pregnancies were established by artificial insemination. Uterine and trophoblast samples were collected on day 35 ±1 of pregnancy, the expression of immune-related genes was examined by qPCR, and the expression of trophoblast microRNAs was assessed by sequencing.<h4>Results</h4>Compared to the control group, trophoblast from MHC-I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL1A, IL2, IL10, IL12B, TBX21, and TNF, while GNLY expression was downregulated. The MHC-I homozygous incompatible treatment group expressed increased levels of IFNG, IL1A, and IL2 while the MHC-I homozygous compatible group did not differentially express any genes compared to the control group. In the endometrium, relative to the control group, MHC-I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL10, IL12B, and TNF, while GATA3 expression was downregulated. The MHC-I homozygous incompatible group expressed decreased amounts of CSF2 transcripts compared with the control group but did not have abnormal expression of any other immune-related genes. MHC-I incompatible pregnancies had 40 deregulated miRNAs compared to control pregnancies and 62 deregulated microRNAs compared to MHC-I compatible pregnancies.<h4>Conclusions</h4>MHC-I compatibility between the dam and fetus prevented an exacerbated maternal immune response from being mounted against fetal antigens."],"journal":["American journal of reproductive immunology (New York, N.Y. : 1989)"],"pubmed_title":["Increased expression of pro-inflammatory cytokines at the fetal-maternal interface in bovine pregnancies produced by cloning."],"pmcid":["PMC9285385"],"funding_grant_id":["R01 HD055502"],"pubmed_authors":["Wilhelm A","Duhan N","White KL","Umbaugh JJ","Davies CJ","Rutigliano HM","Kaundal R","Schlafer DH","Thomas AJ","Sessions BR","Hicks BA"],"additional_accession":[]},"is_claimable":false,"name":"Increased expression of pro-inflammatory cytokines at the fetal-maternal interface in bovine pregnancies produced by cloning.","description":"<h4>Problem</h4>A significant rate of spontaneous abortion is observed in cattle pregnancies produced by somatic cell nuclear transfer (SCNT). Major histocompatibility complex class I (MHC-I) proteins are abnormally expressed on the surface of trophoblast cells from SCNT conceptuses.<h4>Method of study</h4>MHC-I homozygous compatible (n = 9), homozygous incompatible (n = 8), and heterozygous incompatible (n = 5) pregnancies were established by SCNT. Eight control pregnancies were established by artificial insemination. Uterine and trophoblast samples were collected on day 35 ±1 of pregnancy, the expression of immune-related genes was examined by qPCR, and the expression of trophoblast microRNAs was assessed by sequencing.<h4>Results</h4>Compared to the control group, trophoblast from MHC-I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL1A, IL2, IL10, IL12B, TBX21, and TNF, while GNLY expression was downregulated. The MHC-I homozygous incompatible treatment group expressed increased levels of IFNG, IL1A, and IL2 while the MHC-I homozygous compatible group did not differentially express any genes compared to the control group. In the endometrium, relative to the control group, MHC-I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL10, IL12B, and TNF, while GATA3 expression was downregulated. The MHC-I homozygous incompatible group expressed decreased amounts of CSF2 transcripts compared with the control group but did not have abnormal expression of any other immune-related genes. MHC-I incompatible pregnancies had 40 deregulated miRNAs compared to control pregnancies and 62 deregulated microRNAs compared to MHC-I compatible pregnancies.<h4>Conclusions</h4>MHC-I compatibility between the dam and fetus prevented an exacerbated maternal immune response from being mounted against fetal antigens.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2024-11-09T19:02:13.534Z","creation":"2022-08-06T14:40:12.179Z"},"accession":"S-EPMC9285385","cross_references":{"pubmed":["34974639"],"doi":["10.1111/aji.13520"]}}