{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hardwick SA"],"funding":["Hungarian Brain Research Program, The Human Brain Tissue Bank, Semmelweis University","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NICHD NIH HHS","Semmelweis Egyetem","NIA NIH HHS","Howard Hughes Medical Institute","NIMH NIH HHS","NHLBI NIH HHS","JPB Foundation","Department of Health | National Health and Medical Research Council","U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","NINDS NIH HHS","Saint Petersburg State University","Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal","NIDA NIH HHS","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse","Brain and Behavior Research Foundation","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute","U.S. Department of Health & Human Services | NIH | National Institute of Mental Health","Feil Family Foundation","U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke","U.S. Department of Health & Human Services | National Institutes of Health","NIGMS NIH HHS"],"pagination":["1082-1092"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9287170"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(7)"],"pubmed_abstract":["Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics, PCR-based artifact removal, target enrichment and long-read sequencing, SnISOr-Seq increased barcoded, exon-spanning long reads 7.5-fold compared to naive long-read single-nuclei sequencing. We applied SnISOr-Seq to adult human frontal cortex and found that exons associated with autism exhibit coordinated and highly cell-type-specific inclusion. We found two distinct combination patterns: those distinguishing neural cell types, enriched in TSS-exon, exon-polyadenylation-site and non-adjacent exo"],"journal":["Nature biotechnology"],"pubmed_title":["Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue."],"pmcid":["PMC9287170"],"funding_grant_id":["FK128252","K128247","R01MH125956","R01 AG051390","APP1156531","R01 GM135247","R01 HL136520","T32 DA039080","1R01GM135247-01","PURE 73023672","R01AG054214","R01 NS105477","HL136520","U01 DA053625-01","U01 DA053625","1R01NS105477","R01 DA008259","U54 NS100717","28172","RF1 MH121267","P01HD067244","DA08259","R01AG051390","U54NS100717","UM1AI164599","R01 NS123562","R01 AG054214","R01 AG072758","U19 AG062418","P01 HD067244","R56 AI164599","U54NS11717","R01 MH125956","U54 NS117170","U01 DA53625","UM1 AI164559","1RF1MH121267-01","2017-1.2.1-NKP-2017-00002"],"pubmed_authors":["Prjibelski AD","Lanjewar S","Hu W","Tilgner HU","Milner TA","Trojanowski JQ","Joglekar A","Palkovits M","Fedrigo O","Tombacz D","Foord C","Ndhlovu LC","Fan L","Balacco J","Sampson MM","Lee VMY","Smit AB","Sloan SA","Hardwick SA","Jarvis E","Ross ME","Belchikov N","Koopmans F","Boldogkoi Z","Gan L","Mikheenko A","Collier PG","Lucas AB","Luo W","Jarroux J"],"additional_accession":[]},"is_claimable":false,"name":"Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue.","description":"Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics, PCR-based artifact removal, target enrichment and long-read sequencing, SnISOr-Seq increased barcoded, exon-spanning long reads 7.5-fold compared to naive long-read single-nuclei sequencing. We applied SnISOr-Seq to adult human frontal cortex and found that exons associated with autism exhibit coordinated and highly cell-type-specific inclusion. We found two distinct combination patterns: those distinguishing neural cell types, enriched in TSS-exon, exon-polyadenylation-site and non-adjacent exo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-05-09T21:54:25.399Z","creation":"2025-02-19T02:33:08.603Z"},"accession":"S-EPMC9287170","cross_references":{"pubmed":["35256815"],"doi":["10.1038/s41587-022-01231-3"]}}