{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shahryarimorad K"],"funding":["shahid beheshti university","Iran National Science Foundation"],"pagination":["94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9287526"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Recombinant production of mussel foot proteins among marine-inspired proteinaceous adhesive materials has been attracted high attention for medical applications, due to their exceptional versatility potential of hierarchically arranged nanostructures. Various biochemical and proteinous factors such as amyloid CsgA curli protein have been used as a synergistic factor to enhance the constancy of obtained bio-adhesion but their mechanistic interactions have not yet been deeply investigated widely in different pH conditions. To this end, the present study has first sought to assess molecular simulation and prediction by using RosettaFold to predict the 3-dimensional structure of the fused CsgA subunit and the MFP3 protein followed by in vitro verification. It was developed an ensemble of quant"],"journal":["AMB Express"],"pubmed_title":["In silico prediction and in vitro validation of the effect of pH on adhesive behaviour of the fused CsgA-MFP3 protein."],"pmcid":["PMC9287526"],"funding_grant_id":["1399","96016699"],"pubmed_authors":["Shokrgozar MA","Alipour A","Shahsavarani H","Abtahi B","Honar YS","Shahryarimorad K"],"additional_accession":[]},"is_claimable":false,"name":"In silico prediction and in vitro validation of the effect of pH on adhesive behaviour of the fused CsgA-MFP3 protein.","description":"Recombinant production of mussel foot proteins among marine-inspired proteinaceous adhesive materials has been attracted high attention for medical applications, due to their exceptional versatility potential of hierarchically arranged nanostructures. Various biochemical and proteinous factors such as amyloid CsgA curli protein have been used as a synergistic factor to enhance the constancy of obtained bio-adhesion but their mechanistic interactions have not yet been deeply investigated widely in different pH conditions. To this end, the present study has first sought to assess molecular simulation and prediction by using RosettaFold to predict the 3-dimensional structure of the fused CsgA subunit and the MFP3 protein followed by in vitro verification. It was developed an ensemble of quant","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-04-08T10:15:01.275Z","creation":"2024-11-08T18:01:06.765Z"},"accession":"S-EPMC9287526","cross_references":{"pubmed":["35838851"],"doi":["10.1186/s13568-022-01435-5"]}}