biostudies-literatureUnknown13Viurcos-Sanabria RConsejo Nacional de Ciencia y TecnologíaThe contribution of the cellular immune response to the severity of coronavirus disease 2019 (COVID-19) is still uncertain because most evidence comes from patients receiving multiple drugs able to change immune function. Herein, we conducted a prospective cohort study and obtained blood samples from 128 unvaccinated healthy volunteers to examine the <i>in vitro</i> response pattern of CD4+ and CD8+ T cells and monocyte subsets to polyclonal stimuli, including anti-CD3, anti-CD28, poly I:C, severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) recombinant spike S1 protein, and lipopolysaccharide. Then, we started a six-month follow-up and registered 12 participants who got SARS-CoV-2 infection, from whom we retrospectively analyzed the basal immune response pattern of T cells aFrontiers in immunology897995https://www.ebi.ac.uk/biostudies/studies/S-EPMC9289744biostudies-literature<i>In Vitro</i> Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19.PMC9289744Viurcos-Sanabria RViurcos-Sanabria VRizo-Tellez SAEscobedo GRodriguez-Cortes OManjarrez-Reyna ANFlores-Mejia RArroyo-Valerio AGonzalez-Chavez AMendez-Garcia LALeon-Pedroza JISolleiro-Villavicencio HCarrillo-Ruiz JDGonzalez-Sanabria Jfalse<i>In Vitro</i> Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19.The contribution of the cellular immune response to the severity of coronavirus disease 2019 (COVID-19) is still uncertain because most evidence comes from patients receiving multiple drugs able to change immune function. Herein, we conducted a prospective cohort study and obtained blood samples from 128 unvaccinated healthy volunteers to examine the <i>in vitro</i> response pattern of CD4+ and CD8+ T cells and monocyte subsets to polyclonal stimuli, including anti-CD3, anti-CD28, poly I:C, severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) recombinant spike S1 protein, and lipopolysaccharide. Then, we started a six-month follow-up and registered 12 participants who got SARS-CoV-2 infection, from whom we retrospectively analyzed the basal immune response pattern of T cells a2022-01-01T00:00:00Z20222025-08-30T03:13:30.151Z2022-08-09T06:00:32.526ZS-EPMC92897443586023610.3389/fimmu.2022.897995