biostudies-literatureEdwards SDNational Eye InstituteNIBIB NIH HHSNEI NIH HHSOffice of Experimental Program to Stimulate Competitive ResearchNational Institute of General Medical SciencesNIGMS NIH HHS2786-2794https://www.ebi.ac.uk/biostudies/studies/S-EPMC9290187biostudies-literatureUnknown5(6)Injectable hydrogels have previously demonstrated potential as a temporary scaffold for tissue regeneration or as a delivery vehicle for cells, growth factors, or drugs. However, most injectable hydrogel systems lack a microporous structure, preventing host cell migration into the hydrogel interior and limiting spreading and proliferation of encapsulated cells. Herein, an injectable microporous hydrogel assembled from gelatin/gelatin methacryloyl (GelMA) composite microgels is described. Microgels are produced by a water-in-oil emulsion using a gelatin/GelMA aqueous mixture. These microgels show improved thermal stability compared to GelMA-only microgels and benefit from combined photopolymerization using UV irradiation (365 nm) in the presence of a photoinitiator (PI) and enzymatic reactiACS applied bio materialsFast-Curing Injectable Microporous Hydrogel for <i>In Situ</i> Cell Encapsulation.PMC9290187OIA-1757371R21 EB0321341R21EY02795301P20 GM113131R21 EY027953P20GM113131Brown JMJeong KJEdwards SDKim YJBoudreau RDHou SLee YfalseFast-Curing Injectable Microporous Hydrogel for <i>In Situ</i> Cell Encapsulation.Injectable hydrogels have previously demonstrated potential as a temporary scaffold for tissue regeneration or as a delivery vehicle for cells, growth factors, or drugs. However, most injectable hydrogel systems lack a microporous structure, preventing host cell migration into the hydrogel interior and limiting spreading and proliferation of encapsulated cells. Herein, an injectable microporous hydrogel assembled from gelatin/gelatin methacryloyl (GelMA) composite microgels is described. Microgels are produced by a water-in-oil emulsion using a gelatin/GelMA aqueous mixture. These microgels show improved thermal stability compared to GelMA-only microgels and benefit from combined photopolymerization using UV irradiation (365 nm) in the presence of a photoinitiator (PI) and enzymatic reacti2022-01-01T00:00:00Z2022 Jun2025-04-22T02:01:10.673Z2025-04-05T20:12:02.763ZS-EPMC92901873557662210.1021/acsabm.2c00214