{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(7)"],"submitter":["Jiang F"],"pubmed_abstract":["Both natural ginsenoside F2 and unnatural ginsenoside 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM were reported to exhibit anti-tumor activity. Traditional approaches for producing them rely on direct extraction from <i>Panax ginseng</i>, enzymatic catalysis or chemical synthesis, all of which result in low yield and high cost. Metabolic engineering of microbes has been recognized as a green and sustainable biotechnology to produce natural and unnatural products. Hence we engineered the complete biosynthetic pathways of F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM in <i>Saccharomyces cerevisiae via</i> the CRISPR/Cas9 system. The titers of F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM were increased from 1.2 to 21.0 mg/L and from 82.0 to 346.1 mg/L at shake flask level, respectively, by mul"],"journal":["Acta pharmaceutica Sinica. B"],"pagination":["3167-3176"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9293705"],"repository":["biostudies-literature"],"pubmed_title":["Metabolic engineering of yeasts for green and sustainable production of bioactive ginsenosides F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM."],"pmcid":["PMC9293705"],"pubmed_authors":["Li Y","Gong T","Yang J","Zhu P","Jiang F","Chen J","Zhou C","Deng H","Chen T"],"additional_accession":[]},"is_claimable":false,"name":"Metabolic engineering of yeasts for green and sustainable production of bioactive ginsenosides F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM.","description":"Both natural ginsenoside F2 and unnatural ginsenoside 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM were reported to exhibit anti-tumor activity. Traditional approaches for producing them rely on direct extraction from <i>Panax ginseng</i>, enzymatic catalysis or chemical synthesis, all of which result in low yield and high cost. Metabolic engineering of microbes has been recognized as a green and sustainable biotechnology to produce natural and unnatural products. Hence we engineered the complete biosynthetic pathways of F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM in <i>Saccharomyces cerevisiae via</i> the CRISPR/Cas9 system. The titers of F2 and 3<i>β</i>,20<i>S</i>-Di-<i>O</i>-Glc-DM were increased from 1.2 to 21.0 mg/L and from 82.0 to 346.1 mg/L at shake flask level, respectively, by mul","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-19T09:24:58.095Z","creation":"2022-08-08T09:51:40.35Z"},"accession":"S-EPMC9293705","cross_references":{"pubmed":["35865098"],"doi":["10.1016/j.apsb.2022.04.012"]}}