{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gu T"],"funding":["American Cancer Society","NIA NIH HHS","NIDDK NIH HHS","NHLBI NIH HHS","NHGRI NIH HHS","NCI NIH HHS","NINDS NIH HHS","Welch Foundation","U.S. Department of Health & Human Services | National Institutes of Health","NIGMS NIH HHS"],"pagination":["625-636"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9295050"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(5)"],"pubmed_abstract":["DNA methyltransferase 3a (DNMT3A) plays a crucial role during mammalian development. Two isoforms of DNMT3A are differentially expressed from stem cells to somatic tissues, but their individual functions remain largely uncharacterized. Here we report that the long isoform DNMT3A1, but not the short DNMT3A2, is essential for mouse postnatal development. DNMT3A1 binds to and regulates bivalent neurodevelopmental genes in the brain. Strikingly, Dnmt3a1 knockout perinatal lethality could be partially rescued by DNMT3A1 restoration in the nervous system. We further show that the intrinsically disordered N terminus of DNMT3A1 is required for normal development and DNA methylation at DNMT3A1-enriched regions. Mechanistically, a ubiquitin-interacting motif embedded in a putative α-helix within the"],"journal":["Nature genetics"],"pubmed_title":["The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development."],"pmcid":["PMC9295050"],"funding_grant_id":["R01GM112003","R01HG007538","R01DK092883","R01HL134780","RSG-18-043-01-LIB","R01 GM112003","NS071153","P01 AG036695","P30 CA125123","P30 CA016672","R01 HL146852","R01 DK092883","R01 HG007538","R01 CA228140","BE-1913-20190330","R01CA228140","R01HL146852","R01 HL134780","R01CA183252","R01 NS071153","R01 CA183252"],"pubmed_authors":["Gu T","Rosas C","Huang TW","Lin X","Jeong M","Zhou Y","Li W","Guo L","Huang Y","Tovy A","Goodell MA","Deneen B","Hao D","Guzman AG","Woo J"],"additional_accession":[]},"is_claimable":false,"name":"The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development.","description":"DNA methyltransferase 3a (DNMT3A) plays a crucial role during mammalian development. Two isoforms of DNMT3A are differentially expressed from stem cells to somatic tissues, but their individual functions remain largely uncharacterized. Here we report that the long isoform DNMT3A1, but not the short DNMT3A2, is essential for mouse postnatal development. DNMT3A1 binds to and regulates bivalent neurodevelopmental genes in the brain. Strikingly, Dnmt3a1 knockout perinatal lethality could be partially rescued by DNMT3A1 restoration in the nervous system. We further show that the intrinsically disordered N terminus of DNMT3A1 is required for normal development and DNA methylation at DNMT3A1-enriched regions. Mechanistically, a ubiquitin-interacting motif embedded in a putative α-helix within the","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-25T23:36:52.017Z","creation":"2025-04-06T09:24:59.022Z"},"accession":"S-EPMC9295050","cross_references":{"pubmed":["35534561"],"doi":["10.1038/s41588-022-01063-6"]}}