{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chang CT"],"funding":["Ministry of Science and Technology","Academia Sinica&apos;s Stroke Biosignature Project","China Medical University Hospital"],"pagination":["542-550"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9296198"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(2)"],"pubmed_abstract":["High-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylated PON-1 protein expression in HDL, and nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) was applied to identify the amino acid in PON-1 carbamylated. PON-1 enzyme activities were estimated by substrates conversion method. HDL anti-oxidant activity was gauged by fluorescence changes of indicator dye in the presence of H<sub>2</sub>O<sub>2</sub>. Our study results proved that the degree of P"],"journal":["Journal of food and drug analysis"],"pubmed_title":["PON-1 carbamylation is enhanced in HDL of uremia patients."],"pmcid":["PMC9296198"],"funding_grant_id":["DMR-107-121","MOST 106-2113-M-039-001","BM10701010021"],"pubmed_authors":["Chen CJ","Tang FY","Lee CW","Chang CT","Liao HY","Yang CY","Lim YP","Chen FY","Chang CM"],"additional_accession":[]},"is_claimable":false,"name":"PON-1 carbamylation is enhanced in HDL of uremia patients.","description":"High-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylated PON-1 protein expression in HDL, and nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) was applied to identify the amino acid in PON-1 carbamylated. PON-1 enzyme activities were estimated by substrates conversion method. HDL anti-oxidant activity was gauged by fluorescence changes of indicator dye in the presence of H<sub>2</sub>O<sub>2</sub>. Our study results proved that the degree of P","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2025-04-25T23:43:54.655Z","creation":"2024-11-06T18:40:14.723Z"},"accession":"S-EPMC9296198","cross_references":{"pubmed":["30987726"],"doi":["10.1016/j.jfda.2018.09.007"]}}