biostudies-literatureChang CTMinistry of Science and TechnologyAcademia Sinica's Stroke Biosignature ProjectChina Medical University Hospital542-550https://www.ebi.ac.uk/biostudies/studies/S-EPMC9296198biostudies-literatureUnknown27(2)High-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylated PON-1 protein expression in HDL, and nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) was applied to identify the amino acid in PON-1 carbamylated. PON-1 enzyme activities were estimated by substrates conversion method. HDL anti-oxidant activity was gauged by fluorescence changes of indicator dye in the presence of H₂O₂. Our study results proved that the degree of PJournal of food and drug analysisPON-1 carbamylation is enhanced in HDL of uremia patients.PMC9296198DMR-107-121MOST 106-2113-M-039-001BM10701010021Chen CJTang FYLee CWChang CTLiao HYYang CYLim YPChen FYChang CMfalsePON-1 carbamylation is enhanced in HDL of uremia patients.High-density lipoprotein (HDL) carbamylation has been known in uremia patients. Paraoxonase-1 (PON-1) is an important HDL protein responsible for HDL anti-oxidant, arylesterase and lactonase activities. PON-1 carbamylation in uremic HDL has never been explored. We isolated HDL from uremia patients and control healthy subjects for study. Sandwich ELISA was used to estimate carbamylated PON-1 protein expression in HDL, and nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) was applied to identify the amino acid in PON-1 carbamylated. PON-1 enzyme activities were estimated by substrates conversion method. HDL anti-oxidant activity was gauged by fluorescence changes of indicator dye in the presence of H₂O₂. Our study results proved that the degree of P2019-01-01T00:00:00Z2019 Apr2025-04-25T23:43:54.655Z2024-11-06T18:40:14.723ZS-EPMC92961983098772610.1016/j.jfda.2018.09.007