{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang L"],"funding":["National Institute of Allergy and Infectious Diseases","National Center for Research Resources","NICHD NIH HHS","NIAID NIH HHS","U.S. Department of Defense","Weatherhead Presidential Endowment Fund","NIH HHS","National Institute of Child Health and Human Development","NIGMS NIH HHS","Office of Research Infrastructure Programs"],"pagination":["9769803"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9297724"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022"],"pubmed_abstract":["Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites, but can be laborious. Herein, we employed peptide microarrays to map linear peptide epitopes (LPEs) recognized following SARS-CoV-2 infection and vaccination. LPEs detected by nonhuman primate (NHP) and patient IgMs after SARS-CoV-2 infection extensively overlapped, localized to functionally important virus regions, and aligned with reported neutralizing antibody binding sites. Similar LPE overlap occurred after infection and vaccination, with LPE clusters specific to each stimulus, where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function. Vaccine-specific LPEs tended to map to sites known or likely to be affected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein. Mapping LPEs to regions of known functional importance in this manner may accelerate vaccine evaluation and discovery of targets for site-specific therapeutic interventions."],"journal":["Research (Washington, D.C.)"],"pubmed_title":["SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites."],"pmcid":["PMC9297724"],"funding_grant_id":["P51 OD011104","R01 HD103511","R01 HD090927","R01HD103511","R01HD090927","U54 GM104940","W8IXWH1910926","OD011104","R01 AI144168","HHSN272201700033I"],"pubmed_authors":["Liang T","Park H","Hu T","Yang L","Li C","Cao J","Roy CJ","Wang H","Fletcher JK","Zhang L","Zheng W","Li Y","Robinson JE","Huang Z","Wang S","Zhang X","Pierson LM","Feehan AK","Morici LA","Lyon CJ","Bao D","Yu X"],"additional_accession":[]},"is_claimable":false,"name":"SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites.","description":"Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites, but can be laborious. Herein, we employed peptide microarrays to map linear peptide epitopes (LPEs) recognized following SARS-CoV-2 infection and vaccination. LPEs detected by nonhuman primate (NHP) and patient IgMs after SARS-CoV-2 infection extensively overlapped, localized to functionally important virus regions, and aligned with reported neutralizing antibody binding sites. Similar LPE overlap occurred after infection and vaccination, with LPE clusters specific to each stimulus, where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function. Vaccine-specific LPEs tended to map to sites known or likely to be affected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein. Mapping LPEs to regions of known functional importance in this manner may accelerate vaccine evaluation and discovery of targets for site-specific therapeutic interventions.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T11:42:18.186Z","creation":"2025-04-22T16:34:48.105Z"},"accession":"S-EPMC9297724","cross_references":{"pubmed":["35928300"],"doi":["10.34133/2022/9769803"]}}