biostudies-literatureBoccaccio ACompagnia di San Paolo2578-2592https://www.ebi.ac.uk/biostudies/studies/S-EPMC9299152biostudies-literatureUnknown289(9)Membrane asymmetry is important for cellular physiology and established by energy-dependent unidirectional lipid translocases, which have diverse physiological functions in plants. By contrast, the role of phospholipid scrambling (PLS), the passive bidirectional lipid transfer leading to the break-down of membrane asymmetry, is currently still unexplored. The Arabidopsis thaliana genome contains a single gene (At1g73020) with homology to the eukaryotic TMEM16 family of Ca²⁺ -activated phospholipid scramblases. Here, we investigated the protein function of this Arabidopsis homolog. Fluorescent AtTMEM16 fusions localized to the ER both in transiently expressing Arabidopsis protoplasts and HEK293 cells. A putative scrambling domain (SCRD) was identified on the basis of sequence conThe FEBS journalPhospholipid scrambling by a TMEM16 homolog of Arabidopsis thaliana.PMC92991522013.0922Boccaccio AScholz-Starke JPicco CDi Zanni EfalsePhospholipid scrambling by a TMEM16 homolog of Arabidopsis thaliana.Membrane asymmetry is important for cellular physiology and established by energy-dependent unidirectional lipid translocases, which have diverse physiological functions in plants. By contrast, the role of phospholipid scrambling (PLS), the passive bidirectional lipid transfer leading to the break-down of membrane asymmetry, is currently still unexplored. The Arabidopsis thaliana genome contains a single gene (At1g73020) with homology to the eukaryotic TMEM16 family of Ca²⁺ -activated phospholipid scramblases. Here, we investigated the protein function of this Arabidopsis homolog. Fluorescent AtTMEM16 fusions localized to the ER both in transiently expressing Arabidopsis protoplasts and HEK293 cells. A putative scrambling domain (SCRD) was identified on the basis of sequence con2022-01-01T00:00:00Z2022 May2026-04-08T10:20:45.663Z2025-04-04T22:12:28.78ZS-EPMC92991523477568010.1111/febs.16279