biostudies-literatureUnknown8(29)Van Dyke ETZero- to ultralow-field nuclear magnetic resonance (ZULF NMR) is a rapidly developing form of spectroscopy that provides rich spectroscopic information in the absence of large magnetic fields. However, signal acquisition still requires a mechanism for generating a bulk magnetic moment for detection, and the currently used methods only apply to a limited pool of chemicals or come at prohibitively high cost. We demonstrate that the parahydrogen-based SABRE (signal amplification by reversible exchange)-Relay method can be used as a more general means of generating hyperpolarized analytes for ZULF NMR by observing zero-field <i>J</i>-spectra of [¹³C]-methanol, [1-¹³C]-ethanol, and [2-¹³C]-ethanol in both ¹³C-isotopically enriched and natural abundance samples. We explore the magnetic field dependence of the SABRE-Relay efficiency and show the existence of a second maximum at 19.0 ± 0.3 mT. Despite presence of water, SABRE-Relay is used to hyperpolarize ethanol extracted from a store-bought sample of vodka (%<i>P</i>_H ~ 0.1%).Science advanceseabp9242https://www.ebi.ac.uk/biostudies/studies/S-EPMC9299534biostudies-literatureRelayed hyperpolarization for zero-field nuclear magnetic resonance.PMC9299534Sheberstov KFHu YPicazo-Frutos RBudker DEills JBarskiy DAVan Dyke ETfalseRelayed hyperpolarization for zero-field nuclear magnetic resonance.Zero- to ultralow-field nuclear magnetic resonance (ZULF NMR) is a rapidly developing form of spectroscopy that provides rich spectroscopic information in the absence of large magnetic fields. However, signal acquisition still requires a mechanism for generating a bulk magnetic moment for detection, and the currently used methods only apply to a limited pool of chemicals or come at prohibitively high cost. We demonstrate that the parahydrogen-based SABRE (signal amplification by reversible exchange)-Relay method can be used as a more general means of generating hyperpolarized analytes for ZULF NMR by observing zero-field <i>J</i>-spectra of [¹³C]-methanol, [1-¹³C]-ethanol, and [2-¹³C]-ethanol in both ¹³C-isotopically enriched and natural abundance samples. We explore the magnetic field dependence of the SABRE-Relay efficiency and show the existence of a second maximum at 19.0 ± 0.3 mT. Despite presence of water, SABRE-Relay is used to hyperpolarize ethanol extracted from a store-bought sample of vodka (%<i>P</i>_H ~ 0.1%).2022-01-01T00:00:00Z2022 Jul2026-03-27T16:14:15.647Z2025-02-19T02:26:45.857ZS-EPMC92995343585783710.1126/sciadv.abp9242