biostudies-literatureHogenkamp FNRW Strategieprojekt BioSCMinistry of Culture and Science of the German federal state of North Rhine-Westphalia MKWBioeconomy Science Centere202100467https://www.ebi.ac.uk/biostudies/studies/S-EPMC9299732biostudies-literatureUnknown23(1)Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically characterized, with the absorption exhibiting a red-shift. Their differing linkage through ether, carbonate, and carbamate bonds revealed that carbonate and carbamate bonds are convenient. Subsequently, those compounds were successfully applied in vivo for controlling gene expression in E. coli via blue light illumination. Furthermore, benzoate-based expression systems were subjected to light control by establishing a novel photocaged salicylic acid derivative. Besides its synthesis and in vitro characterization, we demonstrate the challenging choice ofChembiochem : a European journal of chemical biologyOptochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems.PMC9299732313/323-400-00213Bitzenhofer NLBier CPietruszka JJaeger KEHilgers FHaase MDrepper THogenkamp FOphoven VBinder DfalseOptochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems.Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically characterized, with the absorption exhibiting a red-shift. Their differing linkage through ether, carbonate, and carbamate bonds revealed that carbonate and carbamate bonds are convenient. Subsequently, those compounds were successfully applied in vivo for controlling gene expression in E. coli via blue light illumination. Furthermore, benzoate-based expression systems were subjected to light control by establishing a novel photocaged salicylic acid derivative. Besides its synthesis and in vitro characterization, we demonstrate the challenging choice of2022-01-01T00:00:00Z2022 Jan2025-04-25T21:51:31.27Z2025-02-19T03:52:49.594ZS-EPMC92997323475094910.1002/cbic.202100467