biostudies-literatureKlaasen SJEuropean Research Council604-609https://www.ebi.ac.uk/biostudies/studies/S-EPMC9300461biostudies-literatureUnknown607(7919)Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis^1-5. Selective pressures then shape distinct aneuploidy and rearrangement patterns-for example, in cancer^6,7-but it is unknown whether initial biases in segregation errors and micronucleation exist for particular chromosomes. Using single-cell DNA sequencing⁸ after an error-prone mitosis in untransformed, diploid cell lines and organoids, we show that chromosomes have different segregation error frequencies that result in non-random aneuploidy landscapes. Isolation and sequencing of single micronuclei from these cells showed that mis-segregating chromosomes frequently also preferentially becoNatureNuclear chromosome locations dictate segregation error frequencies.PMC9300461855158de Vries SGVukusic KRisteski PFoijer FKind JKlaasen SJLens SMAde Luca KLKoprivec ITolic IMGerrits EMKops GJPLStimac VTruong MAvan Jaarsveld RHMarques JFBakker BKodba SfalseNuclear chromosome locations dictate segregation error frequencies.Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis^1-5. Selective pressures then shape distinct aneuploidy and rearrangement patterns-for example, in cancer^6,7-but it is unknown whether initial biases in segregation errors and micronucleation exist for particular chromosomes. Using single-cell DNA sequencing⁸ after an error-prone mitosis in untransformed, diploid cell lines and organoids, we show that chromosomes have different segregation error frequencies that result in non-random aneuploidy landscapes. Isolation and sequencing of single micronuclei from these cells showed that mis-segregating chromosomes frequently also preferentially beco2022-01-01T00:00:00Z2022 Jul2025-04-05T15:05:16.894Z2025-02-19T03:52:57.052ZS-EPMC93004613583150610.1038/s41586-022-04938-0