biostudies-literatureGarcia-Morales LConsejo Nacional de Ciencia y TecnologíaSecretaría de Investigación y Posgrado, Instituto Politécnico NacionalComisión de Operación y Fomento de Actividades Académicas, Instituto Politécnico NacionalEuropean Regional Development Fund907890https://www.ebi.ac.uk/biostudies/studies/S-EPMC9301340biostudies-literatureUnknown12Mycobacteria, like other microorganisms, survive under different environmental variations by expressing an efficient adaptive response, oriented by regulatory elements, such as transcriptional repressors of the TetR family. These repressors in mycobacteria also appear to be related to cholesterol metabolism. In this study, we have evaluated the effect of a fatty acid (oleic-palmitic-stearic)/cholesterol mixture on some phenotypic and genotypic characteristics of a tetR-mutant strain (<i>BCG_2177c</i> mutated gene) of <i>M. bovis</i> BCG, a homologous of <i>Rv2160A</i> of <i>M. tuberculosis</i>. In order to accomplish this, we have analyzed the global gene expression of this strain by RNA-seq and evaluated its neutral-lipid storage capacity and potential to infect macrophages. We have also Frontiers in cellular and infection microbiologyThe Lack of the TetR-Like Repressor Gene <i>BCG</i>_<i>2177c</i> (<i>Rv2160A</i>) May Help Mycobacteria Overcome Intracellular Redox Stress and Survive Longer Inside Macrophages When Surrounded by a Lipid Environment.PMC9301340CB-2015-01-25518120220091PI19/0066620221037Gonzalez-Y-Merchand JAGarcia-Morales LDel Portillo PCerna-Cortes JFAres MARivera-Gutierrez SOtal IGarcia MJMartin CAnzola JMHelguera-Repetto ACMendez-Tenorio AfalseThe Lack of the TetR-Like Repressor Gene <i>BCG</i>_<i>2177c</i> (<i>Rv2160A</i>) May Help Mycobacteria Overcome Intracellular Redox Stress and Survive Longer Inside Macrophages When Surrounded by a Lipid Environment.Mycobacteria, like other microorganisms, survive under different environmental variations by expressing an efficient adaptive response, oriented by regulatory elements, such as transcriptional repressors of the TetR family. These repressors in mycobacteria also appear to be related to cholesterol metabolism. In this study, we have evaluated the effect of a fatty acid (oleic-palmitic-stearic)/cholesterol mixture on some phenotypic and genotypic characteristics of a tetR-mutant strain (<i>BCG_2177c</i> mutated gene) of <i>M. bovis</i> BCG, a homologous of <i>Rv2160A</i> of <i>M. tuberculosis</i>. In order to accomplish this, we have analyzed the global gene expression of this strain by RNA-seq and evaluated its neutral-lipid storage capacity and potential to infect macrophages. We have also 2022-01-01T00:00:00Z20222026-04-12T15:10:55.573Z2025-02-19T02:43:13.501ZS-EPMC93013403587316010.3389/fcimb.2022.907890