{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chung J"],"funding":["HHS | NIH | National Institute of General Medical Sciences (NIGMS)","NIGMS NIH HHS"],"pagination":["e2115129119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9304009"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(29)"],"pubmed_abstract":["Eukaryotic cells contain branched actin networks that are essential for endocytosis, motility, and other key cellular processes. These networks, which are formed by filamentous actin and the Arp2/3 complex, must subsequently be debranched to allow network remodeling and to recycle the Arp2/3 complex. Debranching appears to be catalyzed by two different members of the actin depolymerizing factor homology protein family: cofilin and glial maturation factor (GMF). However, their mechanisms of debranching are only partially understood. Here, we used single-molecule fluorescence imaging of Arp2/3 complex and actin filaments under physiological ionic conditions to observe debranching by GMF and cofilin. We demonstrate that cofilin, like GMF, is an authentic debrancher independent of its filament"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Single-molecule analysis of actin filament debranching by cofilin and GMF."],"pmcid":["PMC9304009"],"funding_grant_id":["R35 GM134895","R35GM134895","R01 GM081648","R01GM081648"],"pubmed_authors":["Goode BL","Gelles J","Chung J"],"additional_accession":[]},"is_claimable":false,"name":"Single-molecule analysis of actin filament debranching by cofilin and GMF.","description":"Eukaryotic cells contain branched actin networks that are essential for endocytosis, motility, and other key cellular processes. These networks, which are formed by filamentous actin and the Arp2/3 complex, must subsequently be debranched to allow network remodeling and to recycle the Arp2/3 complex. Debranching appears to be catalyzed by two different members of the actin depolymerizing factor homology protein family: cofilin and glial maturation factor (GMF). However, their mechanisms of debranching are only partially understood. Here, we used single-molecule fluorescence imaging of Arp2/3 complex and actin filaments under physiological ionic conditions to observe debranching by GMF and cofilin. We demonstrate that cofilin, like GMF, is an authentic debrancher independent of its filament","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-04T09:30:38.962Z","creation":"2025-04-04T09:30:38.962Z"},"accession":"S-EPMC9304009","cross_references":{"pubmed":["35858314"],"doi":["10.1073/pnas.2115129119"]}}