{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Giacometti M"],"funding":["European Research Council","Politecnico di Milano"],"pagination":["1129-1141"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9306751"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(4)"],"pubmed_abstract":["The search for new rapid diagnostic tests for malaria is a priority for developing an efficient strategy to fight this endemic disease, which affects more than 3 billion people worldwide. In this study, we characterize systematically an easy-to-operate lab-on-chip, designed for the magnetophoretic capture of malaria-infected red blood cells (RBCs). The method relies on the positive magnetic susceptibility of infected RBCs with respect to blood plasma. A matrix of nickel posts fabricated in a silicon chip placed face down is aimed at attracting infected cells, while healthy cells sediment on a glass slide under the action of gravity. Using a model of infected RBCs, that is, erythrocytes with methemoglobin, we obtained a capture efficiency of about 70% after 10 min in static conditions. By proper agitation, the capture efficiency reached 85% after just 5 min. Sample preparation requires only a 1:10 volume dilution of whole blood, previously treated with heparin, in a phosphate-buffered solution. Nonspecific attraction of untreated RBCs was not observed in the same time interval."],"journal":["Biotechnology and bioengineering"],"pubmed_title":["On-chip magnetophoretic capture in a model of malaria-infected red blood cells."],"pmcid":["PMC9306751"],"funding_grant_id":["Interdepartmental lab for Live Cell Imaging (LuCId Lab)","G.A. No. 646990 – NICHOID"],"pubmed_authors":["Giuliani E","Raimondi MT","Fiore GB","Piola M","Antinori S","Monticelli M","Milesi F","Ferrari G","Coppadoro LP","Giacometti M","Bertacco R","Jacchetti E"],"additional_accession":[]},"is_claimable":false,"name":"On-chip magnetophoretic capture in a model of malaria-infected red blood cells.","description":"The search for new rapid diagnostic tests for malaria is a priority for developing an efficient strategy to fight this endemic disease, which affects more than 3 billion people worldwide. In this study, we characterize systematically an easy-to-operate lab-on-chip, designed for the magnetophoretic capture of malaria-infected red blood cells (RBCs). The method relies on the positive magnetic susceptibility of infected RBCs with respect to blood plasma. A matrix of nickel posts fabricated in a silicon chip placed face down is aimed at attracting infected cells, while healthy cells sediment on a glass slide under the action of gravity. Using a model of infected RBCs, that is, erythrocytes with methemoglobin, we obtained a capture efficiency of about 70% after 10 min in static conditions. By proper agitation, the capture efficiency reached 85% after just 5 min. Sample preparation requires only a 1:10 volume dilution of whole blood, previously treated with heparin, in a phosphate-buffered solution. Nonspecific attraction of untreated RBCs was not observed in the same time interval.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2024-02-15T15:09:08.181Z","creation":"2022-08-04T07:07:25.395Z"},"accession":"S-EPMC9306751","cross_references":{"pubmed":["34984673"],"doi":["10.1002/bit.28030"]}}