biostudies-literatureMengele AKDeutsche ForschungsgemeinschaftNovo Nordisk Fondene202114842https://www.ebi.ac.uk/biostudies/studies/S-EPMC9306768biostudies-literatureUnknown61(11)During the light-dependent reaction of photosynthesis, green plants couple photoinduced cascades of redox reactions with transmembrane proton translocations to generate reducing equivalents and chemical energy in the form of NADPH (nicotinamide adenine dinucleotide phosphate) and ATP (adenosine triphosphate), respectively. We mimic these basic processes by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli. Upon irradiation with visible light, the interplay of photocatalytic nicotinamide reduction and enzymatic membrane-located respiration leads to the simultaneous formation of two biologically active cofactors, NADH (nicotinamide adenine dinucleotide) and ATP, respectively. This inorganic-biologic hybrid system thus emulates the cofactor delivering function of an active chloroplast.Angewandte Chemie (International ed. in English)Transforming Escherichia coli Proteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis.PMC9306768NNF17SA0031362364549901Eikmanns BJAmthor SRau SWeixler DSeibold GMMengele AKfalseTransforming Escherichia coli Proteomembranes into Artificial Chloroplasts Using Molecular Photocatalysis.During the light-dependent reaction of photosynthesis, green plants couple photoinduced cascades of redox reactions with transmembrane proton translocations to generate reducing equivalents and chemical energy in the form of NADPH (nicotinamide adenine dinucleotide phosphate) and ATP (adenosine triphosphate), respectively. We mimic these basic processes by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli. Upon irradiation with visible light, the interplay of photocatalytic nicotinamide reduction and enzymatic membrane-located respiration leads to the simultaneous formation of two biologically active cofactors, NADH (nicotinamide adenine dinucleotide) and ATP, respectively. This inorganic-biologic hybrid system thus emulates the cofactor delivering function of an active chloroplast.2022-01-01T00:00:00Z2022 Mar2024-10-18T20:17:37.93Z2022-08-02T07:01:45.758ZS-EPMC93067683493284710.1002/anie.202114842