{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suban S"],"funding":["Israel Science Foundation"],"pagination":["218-229"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9306852"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(2)"],"pubmed_abstract":["A biofilm inhibiting mechanism operates in the cyanobacterium Synechococcus elongatus. Here, we demonstrate that the glycosyltransferase homologue, Ogt, participates in the inhibitory process - inactivation of ogt results in robust biofilm formation. Furthermore, a mutational approach shows requirement of the glycosyltransferase activity for biofilm inhibition. This enzyme is necessary for glycosylation of the pilus subunit and for adequate pilus formation. In contrast to wild-type culture in which most cells exhibit several pili, only 25% of the mutant cells are piliated, half of which possess a single pilus. In spite of this poor piliation, natural DNA competence was similar to that of wild-type; therefore, we propose that the unglycosylated pili facilitate DNA transformation. Additional"],"journal":["Environmental microbiology reports"],"pubmed_title":["Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development."],"pmcid":["PMC9306852"],"funding_grant_id":["1406/14","2494/19"],"pubmed_authors":["Sendersky E","Golden SS","Schwarz R","Suban S"],"additional_accession":[]},"is_claimable":false,"name":"Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development.","description":"A biofilm inhibiting mechanism operates in the cyanobacterium Synechococcus elongatus. Here, we demonstrate that the glycosyltransferase homologue, Ogt, participates in the inhibitory process - inactivation of ogt results in robust biofilm formation. Furthermore, a mutational approach shows requirement of the glycosyltransferase activity for biofilm inhibition. This enzyme is necessary for glycosylation of the pilus subunit and for adequate pilus formation. In contrast to wild-type culture in which most cells exhibit several pili, only 25% of the mutant cells are piliated, half of which possess a single pilus. In spite of this poor piliation, natural DNA competence was similar to that of wild-type; therefore, we propose that the unglycosylated pili facilitate DNA transformation. Additional","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-05-09T15:30:50.541Z","creation":"2022-08-04T07:06:29.118Z"},"accession":"S-EPMC9306852","cross_references":{"pubmed":["35172394"],"doi":["10.1111/1758-2229.13050"]}}