biostudies-literaturevan Heerden BNational Institute of General Medical SciencesNIGMS NIH HHSe2107024https://www.ebi.ac.uk/biostudies/studies/S-EPMC9308725biostudies-literatureUnknown18(29)Real-time feedback-driven single-particle tracking (RT-FD-SPT) is a class of techniques in the field of single-particle tracking that uses feedback control to keep a particle of interest in a detection volume. These methods provide high spatiotemporal resolution on particle dynamics and allow for concurrent spectroscopic measurements. This review article begins with a survey of existing techniques and of applications where RT-FD-SPT has played an important role. Each of the core components of RT-FD-SPT are systematically discussed in order to develop an understanding of the trade-offs that must be made in algorithm design and to create a clear picture of the important differences, advantages, and drawbacks of existing approaches. These components are feedback tracking and control, ranging Small (Weinheim an der Bergstrasse, Germany)Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.PMC93087255R01GM117039R01 GM117039Andersson SBvan Heerden BVickers NAKruger TPJfalseReal-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Real-time feedback-driven single-particle tracking (RT-FD-SPT) is a class of techniques in the field of single-particle tracking that uses feedback control to keep a particle of interest in a detection volume. These methods provide high spatiotemporal resolution on particle dynamics and allow for concurrent spectroscopic measurements. This review article begins with a survey of existing techniques and of applications where RT-FD-SPT has played an important role. Each of the core components of RT-FD-SPT are systematically discussed in order to develop an understanding of the trade-offs that must be made in algorithm design and to create a clear picture of the important differences, advantages, and drawbacks of existing approaches. These components are feedback tracking and control, ranging 2022-01-01T00:00:00Z2022 Jul2025-04-26T07:36:00.8Z2025-04-06T12:24:04.177ZS-EPMC93087253575853410.1002/smll.202107024