biostudies-literature00480Unknown24(6)Wu SM<h4>Objective</h4>Long non-coding RNAs (lncRNAs) feature prominently in tumors. Reportedly, lncRNA zinc finger E-box-binding homeobox 2 antisense RNA 1 (ZEB2-AS1) is aberrantly expressed in a variety of tumors. The present study was aimed to explore ZEB2-AS1 functions and determine mechanism in hepatocellular carcinoma (HCC) progression.<h4>Materials and methods</h4>In this experimental study, expressions of <i>ZEB2-AS1</i>, microRNA <i>(miR)-582-5p</i> and forkhead box C1 <i>(FOXC1)</i> mRNA in HCC tissues and cell lines were detected via quantitative reveres transcription polymerase chain reaction (qRT-PCR). After establishing gain- and loss-of-functions models, cell counting kit-8, 5-bromo-2'-deoxyuridine (BrdU), Transwell assays and flow cytometry analysis were conducted to examine HCC cell multiplication, migration, invasion and apoptosis, respectively. The targeted relationship between <i>miR-582- 5p</i> and <i>ZEB2-AS1</i> was verified via dual-luciferase reporter gene assay. Western blot was utilized for detecting FOXC1 expression in HCC cells after selectively regulating <i>ZEB2-AS1</i> and <i>miR-582-5p.</i><h4>Results</h4>In HCC tissues and cells, <i>ZEB2-AS1</i> expression was increased. High ZEB2-AS1 expression was related to relatively large tumor volume, increased tumor-node-metastasis (TNM) stage and positive lymph node metastasis of the patients. <i>ZEB2-AS1</i> overexpression facilitated HCC cell multiplication, migration, invasion and suppressed apoptosis, while <i>ZEB2-AS1</i> knock-down caused the opposite effects. It was also confirmed that <i>ZEB2-AS1</i> could competitively bind with <i>miR-582-5p</i> to repress its expression, and indirectly up-regulate FOXC1 expression level in HCC cells.<h4>Conclusion</h4>The current study revealed that <i>ZEB2-AS1</i> was over-expressed in HCC tissues and cells. It also upregulated <i>(FOXC1)</i>, through sponging <i>miR-582-5p</i>, to promote HCC progression. This provides new perspectives for elucidating the pathogenesis of HCC.Cell journal285-293https://www.ebi.ac.uk/biostudies/studies/S-EPMC9315215biostudies-literatureLong Non-Coding RNA ZEB2-AS1 Promotes Hepatocellular Carcinoma Progression by Regulating The <i>miR-582-5p/FOXC1</i> Axis.PMC9315215Luo QWu SMChen JLiang YXie LTong YY48falseLong Non-Coding RNA ZEB2-AS1 Promotes Hepatocellular Carcinoma Progression by Regulating The <i>miR-582-5p/FOXC1</i> Axis.<h4>Objective</h4>Long non-coding RNAs (lncRNAs) feature prominently in tumors. Reportedly, lncRNA zinc finger E-box-binding homeobox 2 antisense RNA 1 (ZEB2-AS1) is aberrantly expressed in a variety of tumors. The present study was aimed to explore ZEB2-AS1 functions and determine mechanism in hepatocellular carcinoma (HCC) progression.<h4>Materials and methods</h4>In this experimental study, expressions of <i>ZEB2-AS1</i>, microRNA <i>(miR)-582-5p</i> and forkhead box C1 <i>(FOXC1)</i> mRNA in HCC tissues and cell lines were detected via quantitative reveres transcription polymerase chain reaction (qRT-PCR). After establishing gain- and loss-of-functions models, cell counting kit-8, 5-bromo-2'-deoxyuridine (BrdU), Transwell assays and flow cytometry analysis were conducted to examine HCC cell multiplication, migration, invasion and apoptosis, respectively. The targeted relationship between <i>miR-582- 5p</i> and <i>ZEB2-AS1</i> was verified via dual-luciferase reporter gene assay. Western blot was utilized for detecting FOXC1 expression in HCC cells after selectively regulating <i>ZEB2-AS1</i> and <i>miR-582-5p.</i><h4>Results</h4>In HCC tissues and cells, <i>ZEB2-AS1</i> expression was increased. High ZEB2-AS1 expression was related to relatively large tumor volume, increased tumor-node-metastasis (TNM) stage and positive lymph node metastasis of the patients. <i>ZEB2-AS1</i> overexpression facilitated HCC cell multiplication, migration, invasion and suppressed apoptosis, while <i>ZEB2-AS1</i> knock-down caused the opposite effects. It was also confirmed that <i>ZEB2-AS1</i> could competitively bind with <i>miR-582-5p</i> to repress its expression, and indirectly up-regulate FOXC1 expression level in HCC cells.<h4>Conclusion</h4>The current study revealed that <i>ZEB2-AS1</i> was over-expressed in HCC tissues and cells. It also upregulated <i>(FOXC1)</i>, through sponging <i>miR-582-5p</i>, to promote HCC progression. This provides new perspectives for elucidating the pathogenesis of HCC.2022-01-01T00:00:00Z2022 Jun2024-11-12T07:56:48.346Z2022-08-03T06:10:11.326ZS-EPMC93152153589223010.22074/cellj.2022.7963