biostudies-literatureUnknown13(7)Bidoli CDespite a growing number of non-model insect species is being investigated in recent years, a greater understanding of their physiology is prevented by the lack of genomic resources. This is the case of the common European stick insect <i>Bacillus rossius</i> (Rossi, 1788): in this species, some knowledge is available on hemocyte-related defenses, but little is known about the physiological changes occurring in response to natural or experimental challenges. Here, the transcriptional signatures of adult <i>B. rossius</i> hemocytes were investigated after a short-term (2 h) LPS stimulation in vivo: a total of 2191 differentially expressed genes, mostly involved in proteolysis and carbohydrate and lipid metabolic processes, were identified in the de novo assembled transcriptome and in-depth Insects645https://www.ebi.ac.uk/biostudies/studies/S-EPMC9316843biostudies-literatureTranscriptome Analysis Reveals Early Hemocyte Responses upon In Vivo Stimulation with LPS in the Stick Insect <i>Bacillus rossius</i> (Rossi, 1788).PMC9316843Picchietti SMiccoli ABuonocore FFausto AMGerdol MBidoli CScapigliati GfalseTranscriptome Analysis Reveals Early Hemocyte Responses upon In Vivo Stimulation with LPS in the Stick Insect <i>Bacillus rossius</i> (Rossi, 1788).Despite a growing number of non-model insect species is being investigated in recent years, a greater understanding of their physiology is prevented by the lack of genomic resources. This is the case of the common European stick insect <i>Bacillus rossius</i> (Rossi, 1788): in this species, some knowledge is available on hemocyte-related defenses, but little is known about the physiological changes occurring in response to natural or experimental challenges. Here, the transcriptional signatures of adult <i>B. rossius</i> hemocytes were investigated after a short-term (2 h) LPS stimulation in vivo: a total of 2191 differentially expressed genes, mostly involved in proteolysis and carbohydrate and lipid metabolic processes, were identified in the de novo assembled transcriptome and in-depth 2022-01-01T00:00:00Z2022 Jul2026-05-07T03:10:39.779Z2022-08-05T06:16:06.265ZS-EPMC93168433588682110.3390/insects13070645