{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tao X"],"funding":["U.S. Department of Energy"],"pagination":["e0040122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9317963"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(14)"],"pubmed_abstract":["<i>Rhodanobacter</i> has been found as the dominant genus in aquifers contaminated with high concentrations of nitrate and uranium in Oak Ridge, TN, USA. The <i>in situ</i> stimulation of denitrification has been proposed as a potential method to remediate nitrate and uranium contamination. Among the <i>Rhodanobacter</i> species, Rhodanobacter denitrificans strains have been reported to be capable of denitrification and contain abundant metal resistance genes. However, due to the lack of a mutagenesis system in these strains, our understanding of the mechanisms underlying low-pH resistance and the ability to dominate in the contaminated environment remains limited. Here, we developed an in-frame markerless deletion system in two <i>R. denitrificans</i> strains. First, we optimized the grow"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Development of a Markerless Deletion Mutagenesis System in Nitrate-Reducing Bacterium Rhodanobacter denitrificans."],"pmcid":["PMC9317963"],"funding_grant_id":["DE-AC02-05CH11231"],"pubmed_authors":["Liu J","Li Y","Arkin AP","Zhou A","Michael JP","Deutschbauer AM","Tao X","Chakraborty R","Peng M","Kempher ML","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"Development of a Markerless Deletion Mutagenesis System in Nitrate-Reducing Bacterium Rhodanobacter denitrificans.","description":"<i>Rhodanobacter</i> has been found as the dominant genus in aquifers contaminated with high concentrations of nitrate and uranium in Oak Ridge, TN, USA. The <i>in situ</i> stimulation of denitrification has been proposed as a potential method to remediate nitrate and uranium contamination. Among the <i>Rhodanobacter</i> species, Rhodanobacter denitrificans strains have been reported to be capable of denitrification and contain abundant metal resistance genes. However, due to the lack of a mutagenesis system in these strains, our understanding of the mechanisms underlying low-pH resistance and the ability to dominate in the contaminated environment remains limited. Here, we developed an in-frame markerless deletion system in two <i>R. denitrificans</i> strains. First, we optimized the grow","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-06-12T05:48:17.969Z","creation":"2026-06-12T03:08:22.192Z"},"accession":"S-EPMC9317963","cross_references":{"pubmed":["35737807"],"doi":["10.1128/aem.00401-22"]}}