biostudies-literatureOngtanasup TWalailak UniversityNew Strategic Research (P2P) project, Walailak University, ThailandWalailak University, ThailandFCT/MEC (PIDDAC)1835https://www.ebi.ac.uk/biostudies/studies/S-EPMC9318486biostudies-literatureUnknown11(14)PCSK9 is a promising target for developing novel cholesterol-lowering drugs. We developed a recipe that combined molecular docking, GC-MS/MS, and real-time PCR to identify potential PCSK9 inhibitors for herb ratio determination. Three herbs, <i>Carthamus tinctorius</i>, <i>Coscinium fenestratum</i>, and <i>Zingiber officinale,</i> were used in this study. This work aimed to evaluate cholesterol-lowering through a PCSK9 inhibitory mechanism of these three herbs for defining a suitable ratio. Chemical constituents were identified using GC-MS/MS. The PCSK9 inhibitory potential of the compounds was determined using molecular docking, real-time PCR, and Oil red O staining. It has been shown that most of the active compounds of <i>C. fenestratum</i> and <i>Z. officinale</i> inhibit PCSK9 when exPlants (Basel, Switzerland)The Cholesterol-Modulating Effect of the New Herbal Medicinal Recipe from Yellow Vine (<i>Coscinium fenestratum</i> (Goetgh.)), Ginger (<i>Zingiber officinale</i> Roscoe.), and Safflower (<i>Carthamus tinctorius</i> L.) on Suppressing PCSK9 Expression to Upregulate LDLR Expression in HepG2 Cells.PMC9318486UIDP/50011/2020LA/P/0006/2020WU-IRG-65-013UIDB/50011/2020Nissapatorn VJampa OPereira MLWilairatana PWanmasae SEawsakul KPrommee NLimcharoen TPaul AKOngtanasup TNasongkla NfalseThe Cholesterol-Modulating Effect of the New Herbal Medicinal Recipe from Yellow Vine (<i>Coscinium fenestratum</i> (Goetgh.)), Ginger (<i>Zingiber officinale</i> Roscoe.), and Safflower (<i>Carthamus tinctorius</i> L.) on Suppressing PCSK9 Expression to Upregulate LDLR Expression in HepG2 Cells.PCSK9 is a promising target for developing novel cholesterol-lowering drugs. We developed a recipe that combined molecular docking, GC-MS/MS, and real-time PCR to identify potential PCSK9 inhibitors for herb ratio determination. Three herbs, <i>Carthamus tinctorius</i>, <i>Coscinium fenestratum</i>, and <i>Zingiber officinale,</i> were used in this study. This work aimed to evaluate cholesterol-lowering through a PCSK9 inhibitory mechanism of these three herbs for defining a suitable ratio. Chemical constituents were identified using GC-MS/MS. The PCSK9 inhibitory potential of the compounds was determined using molecular docking, real-time PCR, and Oil red O staining. It has been shown that most of the active compounds of <i>C. fenestratum</i> and <i>Z. officinale</i> inhibit PCSK9 when ex2022-01-01T00:00:00Z2022 Jul2026-05-10T03:19:56.021Z2022-08-06T14:41:41.478ZS-EPMC93184863589046910.3390/plants11141835