{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Musabyimana JP"],"funding":["Deutscher Akademischer Austauschdienst","Deutsche Forschungsgemeinschaft","German Academic Exchange Service (DAAD)"],"pagination":["1419"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9320499"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(7)"],"pubmed_abstract":["S-adenosylmethionine synthetase (SAMS) is a key enzyme for the synthesis of the lone methyl donor S-adenosyl methionine (SAM), which is involved in transmethylation reactions and hence required for cellular processes such as DNA, RNA, and histone methylation, but also polyamine biosynthesis and proteostasis. In the human malaria parasite <i>Plasmodium falciparum</i>, <i>Pf</i>SAMS is encoded by a single gene and has been suggested to be crucial for malaria pathogenesis and transmission; however, to date, <i>Pf</i>SAMS has not been fully characterized. To gain deeper insight into the function of <i>Pf</i>SAMS, we generated a conditional gene knockdown (KD) using the <i>glmS</i> ribozyme system. We show that <i>Pf</i>SAMS localizes to the cytoplasm and the nucleus of blood-stage parasites. <"],"journal":["Microorganisms"],"pubmed_title":["<i>Plasmodium falciparum</i> S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins."],"pmcid":["PMC9320499"],"funding_grant_id":["NG170/1-1","PR905/19-1","PhD Stipend JPM","PR905/20-1","PR905/19-1 (SPP 2225); TE599/9-1 (SPP 2225); PR905/20-1; NG170/1-1","TE599/9-1"],"pubmed_authors":["Flammersfeld A","Distler U","Manti J","Musabyimana JP","Ngwa CJ","Berks C","Pradel G","Burda PC","Sassmannshausen J","Tenzer S","Bennink S","Blaschke L"],"additional_accession":[]},"is_claimable":false,"name":"<i>Plasmodium falciparum</i> S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins.","description":"S-adenosylmethionine synthetase (SAMS) is a key enzyme for the synthesis of the lone methyl donor S-adenosyl methionine (SAM), which is involved in transmethylation reactions and hence required for cellular processes such as DNA, RNA, and histone methylation, but also polyamine biosynthesis and proteostasis. In the human malaria parasite <i>Plasmodium falciparum</i>, <i>Pf</i>SAMS is encoded by a single gene and has been suggested to be crucial for malaria pathogenesis and transmission; however, to date, <i>Pf</i>SAMS has not been fully characterized. To gain deeper insight into the function of <i>Pf</i>SAMS, we generated a conditional gene knockdown (KD) using the <i>glmS</i> ribozyme system. We show that <i>Pf</i>SAMS localizes to the cytoplasm and the nucleus of blood-stage parasites. <","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-04-08T11:09:08.468Z","creation":"2022-08-05T06:16:16.742Z"},"accession":"S-EPMC9320499","cross_references":{"pubmed":["35889137"],"doi":["10.3390/microorganisms10071419"]}}