{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Swafford K"],"funding":["National Institutes of Health, National Institutes of General Medical Sciences","NIGMS NIH HHS"],"pagination":["1144"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9321325"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(7)"],"pubmed_abstract":["The aggressive nature of the activated B cell such as (ABC) subtype of diffuse large B cell (DLBCL) is frequently associated with altered B cell Receptor (BCR) signaling through the activation of key components including the scaffolding protein, <i>CARD11</i>. Most inhibitors, such as ibrutinib, target downstream BCR kinases with often modest and temporary responses for DLBCL patients. Here, we pursue an alternative strategy to target the BCR pathway by leveraging a novel DNA secondary structure to repress transcription. We discovered that a highly guanine (G)-rich element within the <i>CARD11</i> promoter forms a stable G-quadruplex (G4) using circular dichroism and polymerase stop biophysical techniques. We then identified a small molecule, naptho(2,1-b)furan-1-ethanol,2-nitro- (NSC37398"],"journal":["Genes"],"pubmed_title":["Targeting a Novel G-Quadruplex in the <i>CARD11</i> Oncogene Promoter with Naptho(2,1-b)furan-1-ethanol,2-nitro- Requires the Nitro Group."],"pmcid":["PMC9321325"],"funding_grant_id":["P20 GM109005","P20GM109005","P20GM121293","P20 GM121293"],"pubmed_authors":["Acharya B","Kendrick S","Saha D","Swafford K","Raney T","Xu YZ","Frett B","McCrury M"],"additional_accession":[]},"is_claimable":false,"name":"Targeting a Novel G-Quadruplex in the <i>CARD11</i> Oncogene Promoter with Naptho(2,1-b)furan-1-ethanol,2-nitro- Requires the Nitro Group.","description":"The aggressive nature of the activated B cell such as (ABC) subtype of diffuse large B cell (DLBCL) is frequently associated with altered B cell Receptor (BCR) signaling through the activation of key components including the scaffolding protein, <i>CARD11</i>. Most inhibitors, such as ibrutinib, target downstream BCR kinases with often modest and temporary responses for DLBCL patients. Here, we pursue an alternative strategy to target the BCR pathway by leveraging a novel DNA secondary structure to repress transcription. We discovered that a highly guanine (G)-rich element within the <i>CARD11</i> promoter forms a stable G-quadruplex (G4) using circular dichroism and polymerase stop biophysical techniques. We then identified a small molecule, naptho(2,1-b)furan-1-ethanol,2-nitro- (NSC37398","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-07T03:11:10.04Z","creation":"2022-08-07T08:15:18.625Z"},"accession":"S-EPMC9321325","cross_references":{"pubmed":["35885931"],"doi":["10.3390/genes13071144"]}}