{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Smith CA"],"funding":["Rosetrees Trust","Versus Arthritis","Medical Research Council","Engineering and Physical Sciences Research Council"],"pagination":["e22314"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9322318"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["36(5)"],"pubmed_abstract":["Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis. Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs). HESC-chondroprogenitors were treated with SIRT1 activator; SRT1720, or inhibitor; EX527, during differentiation. Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN. Unexpectedly, when SIRT1 was activated, while ACAN was enhanced"],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation."],"pmcid":["PMC9322318"],"funding_grant_id":["R20786","EP/L014904/1","MR/K026666","A1984"],"pubmed_authors":["Bates N","Smith CA","Humphreys PA","Cain SA","Kimber SJ","Naven MA","Dvir-Ginzberg M"],"additional_accession":[]},"is_claimable":false,"name":"SIRT1 activity orchestrates ECM expression during hESC-chondrogenic differentiation.","description":"Epigenetic modification is a key driver of differentiation, and the deacetylase Sirtuin1 (SIRT1) is an established regulator of cell function, ageing, and articular cartilage homeostasis. Here we investigate the role of SIRT1 during development of chondrocytes by using human embryonic stem cells (hESCs). HESC-chondroprogenitors were treated with SIRT1 activator; SRT1720, or inhibitor; EX527, during differentiation. Activation of SIRT1 early in 3D-pellet culture led to significant increases in the expression of ECM genes for type-II collagen (COL2A1) and aggrecan (ACAN), and chondrogenic transcription factors SOX5 and ARID5B, with SOX5 ChIP analysis demonstrating enrichment on the chondrocyte specific -10 (A1) enhancer of ACAN. Unexpectedly, when SIRT1 was activated, while ACAN was enhanced","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-27T03:24:41.635Z","creation":"2025-04-06T18:47:04.052Z"},"accession":"S-EPMC9322318","cross_references":{"pubmed":["35416346"],"doi":["10.1096/fj.202200169R"]}}