biostudies-literatureUnknown67(10)Kusel KAn anaerobic, H(2)-utilizing bacterium, strain RD-1, was isolated from the highest growth-positive dilution series of a root homogenate prepared from the sea grass Halodule wrightii. Cells of RD-1 were gram-positive, spore-forming, motile rods that were linked by connecting filaments. Acetate was produced in stoichiometries indicative of an acetyl coenzyme A (acetyl-CoA) pathway-dependent metabolism when RD-1 utilized H(2)-CO(2), formate, lactate, or pyruvate. Growth on sugars or ethylene glycol yielded acetate and ethanol as end products. RD-1 grew at the expense of glucose in the presence of low initial concentrations (up to 6% [vol/vol]) of O(2) in the headspace of static, horizontally incubated culture tubes; the concentration of O(2) decreased during growth in such cultures. PeroxidasApplied and environmental microbiology4734-41https://www.ebi.ac.uk/biostudies/studies/S-EPMC93226biostudies-literaturePhysiological ecology of Clostridium glycolicum RD-1, an aerotolerant acetogen isolated from sea grass roots.PMC93226Karnholz AKusel KAcker GDevereux RDrake HLTrinkwalter TfalsePhysiological ecology of Clostridium glycolicum RD-1, an aerotolerant acetogen isolated from sea grass roots.An anaerobic, H(2)-utilizing bacterium, strain RD-1, was isolated from the highest growth-positive dilution series of a root homogenate prepared from the sea grass Halodule wrightii. Cells of RD-1 were gram-positive, spore-forming, motile rods that were linked by connecting filaments. Acetate was produced in stoichiometries indicative of an acetyl coenzyme A (acetyl-CoA) pathway-dependent metabolism when RD-1 utilized H(2)-CO(2), formate, lactate, or pyruvate. Growth on sugars or ethylene glycol yielded acetate and ethanol as end products. RD-1 grew at the expense of glucose in the presence of low initial concentrations (up to 6% [vol/vol]) of O(2) in the headspace of static, horizontally incubated culture tubes; the concentration of O(2) decreased during growth in such cultures. Peroxidas2001-01-01T00:00:00Z2001 Oct2025-04-05T07:10:39.981Z2019-03-27T00:18:30ZS-EPMC932261157117910.1128/aem.67.10.4734-4741.200110.1128/AEM.67.10.4734-4741.2001