biostudies-literatureUnknown13(7)Feng JTo further understand the origin and evolution of Patellogastropoda, we determined the mitochondrial genome sequence of <i>Cellana toreuma</i>, and compared its mitogenome characteristics with the other four limpets of Nacellidae. The ratio of Ka and Ks indicated that these Nacellidae species were suffering a purifying selection, with exception of the <i>atp6</i> gene. The gene sequence is basically consistent among families, while there are great differences among Lottidae species. According to the mitogenome sequences of selected gastropod species, we reconstructed a new phylogenetic tree with two methods. The data complement the mitogenome database of limpets and is a favorable research tool for the phylogenetic analysis of Gastropoda. It is found that there is a long-branch attraction Genes1273https://www.ebi.ac.uk/biostudies/studies/S-EPMC9322768biostudies-literatureInsights into the Deep Phylogeny and Novel Divergence Time Estimation of Patellogastropoda from Complete Mitogenomes.PMC9322768Xu KLi JFeng JYe YGuo BYan XMiao JfalseInsights into the Deep Phylogeny and Novel Divergence Time Estimation of Patellogastropoda from Complete Mitogenomes.To further understand the origin and evolution of Patellogastropoda, we determined the mitochondrial genome sequence of <i>Cellana toreuma</i>, and compared its mitogenome characteristics with the other four limpets of Nacellidae. The ratio of Ka and Ks indicated that these Nacellidae species were suffering a purifying selection, with exception of the <i>atp6</i> gene. The gene sequence is basically consistent among families, while there are great differences among Lottidae species. According to the mitogenome sequences of selected gastropod species, we reconstructed a new phylogenetic tree with two methods. The data complement the mitogenome database of limpets and is a favorable research tool for the phylogenetic analysis of Gastropoda. It is found that there is a long-branch attraction 2022-01-01T00:00:00Z2022 Jul2025-04-26T13:04:01.355Z2025-02-19T04:38:04.412ZS-EPMC93227683588605610.3390/genes13071273