biostudies-literatureBorges MMBCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCapes scholarship758-771https://www.ebi.ac.uk/biostudies/studies/S-EPMC9325035biostudies-literatureUnknown55(7)<h4>Aim</h4>The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2% and 5% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow.<h4>Methodology</h4>Dual-species biofilms comprised of StreptococcInternational endodontic journalThe response of dual-species bacterial biofilm to 2% and 5% NaOCl mixed with etidronic acid: A laboratory real-time evaluation using optical coherence tomography.PMC932503588887.371170/2019-0088887.371170/2019‐00Petridis XBorges MMBDuarte MAHDijkstra RJBvan der Sluis LWMVersluis Mde Andrade FBfalseThe response of dual-species bacterial biofilm to 2% and 5% NaOCl mixed with etidronic acid: A laboratory real-time evaluation using optical coherence tomography.<h4>Aim</h4>The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2% and 5% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow.<h4>Methodology</h4>Dual-species biofilms comprised of Streptococc2022-01-01T00:00:00Z2022 Jul2025-04-18T15:37:10.668Z2025-04-07T02:21:04.468ZS-EPMC93250353547043410.1111/iej.13754