biostudies-literatureCeron-Chafla PHorizon 2020 Framework ProgrammeFonds Wetenschappelijk OnderzoekUniversiteit Gent1792-1807https://www.ebi.ac.uk/biostudies/studies/S-EPMC9325544biostudies-literatureUnknown119(7)Fermentation at elevated hydrostatic pressure is a novel strategy targeting product selectivity. However, the role of inoculum history and cross-resistance, that is, acquired tolerance from incubation under distinctive environmental stress, remains unclear in high-pressure operation. In our here presented work, we studied fermentation and microbial community responses of halotolerant marine sediment inoculum (MSI) and anaerobic digester inoculum (ADI), pre-incubated in serum bottles at different temperatures and subsequently exposed to mild hydrostatic pressure (MHP; < 10 MPa) in stainless steel reactors. Results showed that MHP effects on microbial growth, activity, and community structure were strongly temperature-dependent. At moderate temperature (20°C), biomass yield and fermentation Biotechnology and bioengineeringPre-incubation conditions determine the fermentation pattern and microbial community structure in fermenters at mild hydrostatic pressure.PMC9325544G020119N676070 (SuPER‐W)(BOF15/GOA/006)de Vrieze JRabaey Kvan Lier JBCeron-Chafla PLindeboom REFGarcia-Timermans CBoon NGanigue RfalsePre-incubation conditions determine the fermentation pattern and microbial community structure in fermenters at mild hydrostatic pressure.Fermentation at elevated hydrostatic pressure is a novel strategy targeting product selectivity. However, the role of inoculum history and cross-resistance, that is, acquired tolerance from incubation under distinctive environmental stress, remains unclear in high-pressure operation. In our here presented work, we studied fermentation and microbial community responses of halotolerant marine sediment inoculum (MSI) and anaerobic digester inoculum (ADI), pre-incubated in serum bottles at different temperatures and subsequently exposed to mild hydrostatic pressure (MHP; < 10 MPa) in stainless steel reactors. Results showed that MHP effects on microbial growth, activity, and community structure were strongly temperature-dependent. At moderate temperature (20°C), biomass yield and fermentation 2022-01-01T00:00:00Z2022 Jul2026-03-31T11:27:38.403Z2024-10-19T04:19:26.434ZS-EPMC93255443531206510.1002/bit.28085