{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ejdrup AL"],"funding":["Lundbeckfonden","Lundbeck Foundation"],"pagination":["4388"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9334352"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by current analysis tools that often reduce colocalization to a single value. Here, we describe a tool specifically designed for determination of co-localization in both 2D and 3D from SMLM data. The approach uses a function that describes the relative enrichment of one molecular species on the density distribution of a reference species. The function reframes the question of colocalization by providing a density-context relevant to multiple biological questions. Moreover, the function visualize enrichment (i.e. colocalization) directly in the ima"],"journal":["Nature communications"],"pubmed_title":["A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data."],"pmcid":["PMC9334352"],"funding_grant_id":["R230-2016-3154","R303-2018-3540","R276-2018-792","R266-2017-4331"],"pubmed_authors":["Madsen KL","Gether U","Konomi A","Herborg F","Ejdrup AL","Lycas MD","Lorenzen N"],"additional_accession":[]},"is_claimable":false,"name":"A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.","description":"Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by current analysis tools that often reduce colocalization to a single value. Here, we describe a tool specifically designed for determination of co-localization in both 2D and 3D from SMLM data. The approach uses a function that describes the relative enrichment of one molecular species on the density distribution of a reference species. The function reframes the question of colocalization by providing a density-context relevant to multiple biological questions. Moreover, the function visualize enrichment (i.e. colocalization) directly in the ima","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-19T11:15:45.338Z","creation":"2025-04-19T11:15:45.338Z"},"accession":"S-EPMC9334352","cross_references":{"pubmed":["35902578"],"doi":["10.1038/s41467-022-32064-y"]}}