{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tetreau G"],"funding":["U.S. Department of Health & Human Services | Centers for Disease Control and Prevention","Agence Nationale de la Recherche","NCRR NIH HHS","ACL HHS","Centre National de la Recherche Scientifique","DOE | Office of Science","U.S. Department of Health & Human Services | National Institutes of Health","NIGMS NIH HHS","NCEZID CDC HHS"],"pagination":["4376"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9334358"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable cry"],"journal":["Nature communications"],"pubmed_title":["De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals."],"pmcid":["PMC9334358"],"funding_grant_id":["1U01CK000516","U01 CK000516","P41GM103393","P41RR001209","ANR-17-EURE-0003","ANR-10-INBS-05-02","P41 RR001209","DE-AC02-76SF00515","R01 GM117126","P41 GM103393","T32 GM007185","PEPS SASLELX","NR-17-CE11-0018-01","GM117126","U01CK000516","ANR-2018-CE11-0005-02"],"pubmed_authors":["Federici B","Doak RB","Bielecki J","Weik M","Schubert R","Canfield EJ","Kloos M","Hilpert M","Grunbein ML","Cascio D","Park HW","Shoeman RL","Riobe F","Brewster AS","Maury O","Schlichting I","Roux A","Teulon JM","De Zitter E","Banneville AS","Engilberge S","Bacia M","Burghammer M","de Wijn R","Tetreau G","Kovacs GN","Laporte F","Zala N","Qiao P","Pellequer JL","Coquelle N","Mancuso A","Young ID","Gutsche I","Batyuk A","Rodriguez J","Gorel A","Barends TRM","Despres L","Sauter NK","Colletier JP","Schibrowsky NA","Signor L","Ling WL","Bean R","Sierra RG","Alba MM","Sawaya MR","Schiro G","Snigireva I","Andreeva EA","Hunter MS","Fenel D","Sato T","Stricker M","Boutet S","Chapelle C","Foucar L","Kirkwood H","Letrun R","Laganowsky AD","Bideshi D","Girard E"],"additional_accession":[]},"is_claimable":false,"name":"De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals.","description":"Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable cry","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2026-05-28T01:11:30.67Z","creation":"2025-04-19T22:47:56.564Z"},"accession":"S-EPMC9334358","cross_references":{"pubmed":["35902572"],"doi":["10.1038/s41467-022-31746-x"]}}