biostudies-literatureUnknown35(4)Chang SDThis study empirically assessed the strength and duration of short-term effects induced by brain reactions to closing/opening the eyes on a few well-known resting-state networks. We also examined the association between these reactions and subjects' cortisol levels. A total of 55 young adults underwent 8-min resting-state fMRI (rs-fMRI) scans under 4-min eyes-closed and 4-min eyes-open conditions. Saliva samples were collected from 25 of the 55 subjects before and after the fMRI sessions and assayed for cortisol levels. Our empirical results indicate that when the subjects were relaxed with their eyes closed, the effect of opening the eyes on conventional resting-state networks (e.g., default-mode, frontal-parietal, and saliency networks) lasted for roughly 60-s, during which we observed aBrain topography375-397https://www.ebi.ac.uk/biostudies/studies/S-EPMC9334428biostudies-literatureBrain Reactions to Opening and Closing the Eyes: Salivary Cortisol and Functional Connectivity.PMC9334428Kuo PCCheng PETsai ACChang SDDuong TQHuang ACWEickhoff SBZilles KLiou MfalseBrain Reactions to Opening and Closing the Eyes: Salivary Cortisol and Functional Connectivity.This study empirically assessed the strength and duration of short-term effects induced by brain reactions to closing/opening the eyes on a few well-known resting-state networks. We also examined the association between these reactions and subjects' cortisol levels. A total of 55 young adults underwent 8-min resting-state fMRI (rs-fMRI) scans under 4-min eyes-closed and 4-min eyes-open conditions. Saliva samples were collected from 25 of the 55 subjects before and after the fMRI sessions and assayed for cortisol levels. Our empirical results indicate that when the subjects were relaxed with their eyes closed, the effect of opening the eyes on conventional resting-state networks (e.g., default-mode, frontal-parietal, and saliency networks) lasted for roughly 60-s, during which we observed a2022-01-01T00:00:00Z2022 Jul2025-04-18T22:19:31.894Z2024-10-19T04:19:34.628ZS-EPMC93344283566636410.1007/s10548-022-00897-x