{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Leupold L"],"pubmed_abstract":["The accumulation of alpha-synuclein (aSyn) is the hallmark of a group of neurodegenerative conditions termed synucleopathies. Physiological functions of aSyn, including those outside of the CNS, remain elusive. However, a reliable and reproducible evaluation of aSyn protein expression in different cell types and especially in low-expressing cells is impeded by the existence of a huge variety of poorly characterized anti-aSyn antibodies and a lack of a routinely used sensitive detection methods. Here, we developed a robust flow cytometry-based workflow for aSyn detection and antibody validation. We test our workflow using three commercially available antibodies (MJFR1, LB509, and 2A7) in a variety of human cell types, including induced pluripotent stem cells, T lymphocytes, and fibroblasts,"],"journal":["Frontiers in neurology"],"pagination":["869103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9334871"],"repository":["biostudies-literature"],"pubmed_title":["The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis."],"pmcid":["PMC9334871"],"pubmed_authors":["Leupold L","Regensburger M","Winner B","Zundler S","Sigutova V","Prots I","Zunke F","Xiang W","Gerasimova E"],"additional_accession":[]},"is_claimable":false,"name":"The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.","description":"The accumulation of alpha-synuclein (aSyn) is the hallmark of a group of neurodegenerative conditions termed synucleopathies. Physiological functions of aSyn, including those outside of the CNS, remain elusive. However, a reliable and reproducible evaluation of aSyn protein expression in different cell types and especially in low-expressing cells is impeded by the existence of a huge variety of poorly characterized anti-aSyn antibodies and a lack of a routinely used sensitive detection methods. Here, we developed a robust flow cytometry-based workflow for aSyn detection and antibody validation. We test our workflow using three commercially available antibodies (MJFR1, LB509, and 2A7) in a variety of human cell types, including induced pluripotent stem cells, T lymphocytes, and fibroblasts,","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-27T01:10:35.146Z","creation":"2025-04-06T18:09:36.954Z"},"accession":"S-EPMC9334871","cross_references":{"pubmed":["35911883"],"doi":["10.3389/fneur.2022.869103"]}}