biostudies-literatureUnknown14(1)Hogg SJ<h4>Background</h4>Interferon gamma (IFNγ) is a pro-inflammatory cytokine that directly activates the JAK/STAT pathway. However, the temporal dynamics of chromatin remodeling and transcriptional activation initiated by IFNγ have not been systematically profiled in an unbiased manner. Herein, we integrated transcriptomic and epigenomic profiling to characterize the acute epigenetic changes induced by IFNγ stimulation in a murine breast cancer model.<h4>Results</h4>We identified de novo activation of cis-regulatory elements bound by Irf1 that were characterized by increased chromatin accessibility, differential usage of pro-inflammatory enhancers, and downstream recruitment of BET proteins and RNA polymerase II. To functionally validate this hierarchical model of IFNγ-driven transcription, wClinical epigenetics96https://www.ebi.ac.uk/biostudies/studies/S-EPMC9336046biostudies-literatureDistinct modulation of IFNγ-induced transcription by BET bromodomain and catalytic P300/CBP inhibition in breast cancer.PMC9336046Zethoven MKearney CJLai AVervoort SJJohnstone RWTodorovski IHouse IGDerrick EBShortt JHogg SJMotorna OBromberg KDKelly MJBeavis PAfalseDistinct modulation of IFNγ-induced transcription by BET bromodomain and catalytic P300/CBP inhibition in breast cancer.<h4>Background</h4>Interferon gamma (IFNγ) is a pro-inflammatory cytokine that directly activates the JAK/STAT pathway. However, the temporal dynamics of chromatin remodeling and transcriptional activation initiated by IFNγ have not been systematically profiled in an unbiased manner. Herein, we integrated transcriptomic and epigenomic profiling to characterize the acute epigenetic changes induced by IFNγ stimulation in a murine breast cancer model.<h4>Results</h4>We identified de novo activation of cis-regulatory elements bound by Irf1 that were characterized by increased chromatin accessibility, differential usage of pro-inflammatory enhancers, and downstream recruitment of BET proteins and RNA polymerase II. To functionally validate this hierarchical model of IFNγ-driven transcription, w2022-01-01T00:00:00Z2022 Jul2026-05-13T14:35:56.568Z2025-02-19T03:29:33.916ZS-EPMC93360463590288610.1186/s13148-022-01316-5