{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li M"],"funding":["National Key Research and Development Program of China","Heilongjiang Bayi Agricultural University"],"pagination":["944390"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9336541"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["Early weaning increased the economic benefits of piglets. However, early weaning damages the intestinal barrier of piglets and causes immunological stress. The mechanism by which <i>Hippophae rhamnoides</i> polysaccharide (HRP) alleviates lipopolysaccharide (LPS)-induced intestinal porcine epithelial cells (IPEC-J2) inflammatory damage was investigated using proteomics in our previous studies. In this study we employed RNA-sequencing (RNA-seq) to determine the level and function of differentially expressed genes (DEGs) and further explore the mechanism of the HRP anti-inflammatory and immune process. The differential expression analysis indicated that 3622, 1216, and 2100 DEGs in the IPEC-J2 cells were identified in C vs. L, L vs. H6-L, and C vs. H6-L, respectively. The Kyoto Encyclopedia "],"journal":["Frontiers in nutrition"],"pubmed_title":["Research on the Mechanism of HRP Relieving IPEC-J2 Cells Immunological Stress Based on Transcriptome Sequencing Analysis."],"pmcid":["PMC9336541"],"funding_grant_id":["NO. XYB202015","2017YFD0500506"],"pubmed_authors":["Zhao L","Chen L","Sun H","Li M","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Research on the Mechanism of HRP Relieving IPEC-J2 Cells Immunological Stress Based on Transcriptome Sequencing Analysis.","description":"Early weaning increased the economic benefits of piglets. However, early weaning damages the intestinal barrier of piglets and causes immunological stress. The mechanism by which <i>Hippophae rhamnoides</i> polysaccharide (HRP) alleviates lipopolysaccharide (LPS)-induced intestinal porcine epithelial cells (IPEC-J2) inflammatory damage was investigated using proteomics in our previous studies. In this study we employed RNA-sequencing (RNA-seq) to determine the level and function of differentially expressed genes (DEGs) and further explore the mechanism of the HRP anti-inflammatory and immune process. The differential expression analysis indicated that 3622, 1216, and 2100 DEGs in the IPEC-J2 cells were identified in C vs. L, L vs. H6-L, and C vs. H6-L, respectively. The Kyoto Encyclopedia ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T10:07:12.595Z","creation":"2025-04-19T22:47:33.729Z"},"accession":"S-EPMC9336541","cross_references":{"pubmed":["35911118"],"doi":["10.3389/fnut.2022.944390"]}}