{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nudell V"],"funding":["NIDDK NIH HHS","NIMH NIH HHS","NINDS NIH HHS","Donald E. and Delia B. Baxter Foundation","Dana Foundation","Harold L. Dorris Neuroscience Foundation","NIGMS NIH HHS"],"pagination":["479-485"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9337799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(4)"],"pubmed_abstract":["The recent development of solvent- and polymer-based brain-clearing techniques has advanced our ability to visualize the mammalian nervous system in three dimensions. However, it remains challenging to image the mammalian body en bloc. Here we developed HYBRiD (hydrogel-based reinforcement of three-dimensional imaging solvent-cleared organs (DISCO)), by recombining components of organic- and polymer-based clearing pipelines. We achieved high transparency and protein retention, as well as compatibility with direct fluorescent imaging and immunostaining in cleared mammalian bodies. Using parvalbumin- and somatostatin-Cre models, we demonstrated the utility of HYBRiD for whole-body imaging of genetically encoded fluorescent reporters without antibody enhancement of signals in newborn and juve"],"journal":["Nature methods"],"pubmed_title":["HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies."],"pmcid":["PMC9337799"],"funding_grant_id":["R01 GM117049","K01 DK114165","R03 DK124731","R01 MH118442","R01 NS087026","DP2 DK128800","RF1 MH123224"],"pubmed_authors":["Teijaro J","Ye L","Maximov A","Nudell V","Huang M","Pang Z","Kanim W","Wang Y","Shaabani N","Lal NK"],"additional_accession":[]},"is_claimable":false,"name":"HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.","description":"The recent development of solvent- and polymer-based brain-clearing techniques has advanced our ability to visualize the mammalian nervous system in three dimensions. However, it remains challenging to image the mammalian body en bloc. Here we developed HYBRiD (hydrogel-based reinforcement of three-dimensional imaging solvent-cleared organs (DISCO)), by recombining components of organic- and polymer-based clearing pipelines. We achieved high transparency and protein retention, as well as compatibility with direct fluorescent imaging and immunostaining in cleared mammalian bodies. Using parvalbumin- and somatostatin-Cre models, we demonstrated the utility of HYBRiD for whole-body imaging of genetically encoded fluorescent reporters without antibody enhancement of signals in newborn and juve","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-19T11:16:37.571Z","creation":"2025-04-19T11:16:37.571Z"},"accession":"S-EPMC9337799","cross_references":{"pubmed":["35347322"],"doi":["10.1038/s41592-022-01427-0"]}}